MSACL 2016 US Abstract

Development and Applications of Sorbent-attached Membrane Funnel Spray Mass Spectrometry for Analysis of Biological Samples

Tak Wah Dominic Chan (Presenter)
The Chinese University of Hong Kong

Authorship: Dominic T.W. Chan, Hoi Sze Yeung, Wan Li, Winnie Hung, Xiangfeng Chen
Department of Chemistry, The Chinese University of Hong Kong, Hong Kong

Short Abstract

Sorbent-attached membrane funnel spray ionization method was a novel mass spectrometry technique for high sensitive, low cost, high throughput analysis of complex samples using in-situ solid-phase extraction cleanup process prior to membrane funnel spray ionization. The sample platform was made from low-cost disposable materials, such as Parafilm, and could easily accommodate tens to hundreds of samples. The sample deposition, cleanup and spray analysis could be automated. The method could also be used to generate chemical images from imprints developed on the sorbent-attached membrane. With multiple washings using solvents with increasing solvent strength, imaging of low-abundant or low polarity chemicals could also be obtained.

Long Abstract

Sorbent-attached membrane funnel, which permits in situ clean-up prior to membrane funnel-based spray analysis, was developed. Fabrication of the membrane funnels and the mounting of SPE sorbent were simple and straightforward by homemade punching system. Using different sorbents, the SPE sorbent-attached funnel has been successfully applied in spray analysis of drug molecules spiked in human plasma, trypsin digest of bovine serum albumin and selective detection of phosphopeptides in the trypsin digest of beta-casein. Using some anti-diabetic drugs as test samples, it was demonstrated that 65-85% of drug analytes could be retrieved from the human plasma matrix after performing solid phase extraction. The sensitivity was excellent and the limit of detection for five target drugs spiked in human plasma was around 0.3-3.2 ng/mL. By applying an isotope labeled internal standard, the signal fluctuation caused by residual background matrices was largely alleviated and the precision of measurement (RSD) was below 15%. Good linearity (R2 > 0.99) was obtained in concentration range of 0.45-90.6 ng/mL. In average, it only took about 30 s for both the in-situ solid phase extraction procedure and MS analysis. Sorbent-attached membrane funnel spray ionization method was also used to image chemicals in biological tissue/section. An imprint of a biological section, e.g. strawberry section, could be formed using a 2D array of membrane funnels. In-situ SPE process on the sorbent-attached funnels could alleviate matrix-suppression effect and make the imaging of low-abundant or low polarity chemicals possible.

References & Acknowledgements:

1. H. S. Yeung, W. Li, Z. Wang, Y. L. E. Wong, X. Chen and T.-W. D. Chan, Rapid Commun. Mass Spectrom., 2015, 29, 336.

2.H. S. Yeung, X. Chen, W. Li, Z. Wang, Y. L. E. Wong, and T.-W. D. Chan, Anal. Chem., 2015, 87, 3149.

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