MSACL 2016 US Abstract

Matrix-free Imaging of Metabolites and Lipids in Tissues and Bacterial Colonies by LAESI and NAPA Mass Spectrometry

Akos Vertes (Presenter)
George Washington University

Bio: Akos Vertes, Ph.D., D.Sc., is a Professor of Chemistry and a Professor of Biochemistry and Molecular Biology at The George Washington University in Washington, DC, USA. His research interests include the development of new analytical technologies applicable in diverse areas of chemistry, biology and medicine. His laboratory develops new methods for metabolic imaging of biological tissues under native conditions, and the in situ interrogation of single cells and subcellular compartments. His interdisciplinary research has been presented in over 160 peer-reviewed publications and in two edited books. He was elected Fellow of the National Academy of Inventors, and received the 2012 Hillebrand Prize and the Oscar and Shoshana Trachtenberg Prize for Scholarship. He is a Doctor of the Hungarian Academy of Sciences. His inventions garnered 15 issued patents, a “Top 10 Innovations” award from The Scientist magazine in the UK, and an “R&D 100 Award” from the R&D Magazine.

Authorship: Akos Vertes
George Washington University, Washington, DC, USA

Short Abstract Spatial distributions of metabolites and lipids in biological tissues have been studied for decades by matrix-assisted laser desorption ionization mass spectrometry and secondary ion mass spectrometry. Despite the success of these techniques characterized by high mass capabilities and excellent spatial resolution, respectively, new ionization sources are being sought to provide better quantitation and fewer spectral interferences in the low mass region. Laser ablation electrospray ionization (LAESI) enables the two- and three-dimensional molecular imaging of tissues and bacterial colonies under ambient conditions. Thin tissue sections and subcellular structures (e.g., lamellipodia) on silicon nanopost array (NAPA) platforms are analyzed and imaged by laser desorption ionization mass spectrometry.

Long Abstract

Spatial distributions of metabolites and lipids in biological tissues have been studied for decades by matrix-assisted laser desorption ionization mass spectrometry and secondary ion mass spectrometry. Despite the success of these techniques characterized by high mass capabilities and excellent spatial resolution, respectively, new ionization sources are being sought to provide better quantitation and fewer spectral interferences in the low mass region. Laser ablation electrospray ionization (LAESI) enables the two- and three-dimensional molecular imaging of tissues and bacterial colonies under ambient conditions. Thin tissue sections and subcellular structures (e.g., lamellipodia) on silicon nanopost array (NAPA) platforms are analyzed and imaged by laser desorption ionization mass spectrometry.

References & Acknowledgements:

Financial Disclosure

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IP Desc:Protea Biosciences, Inc.

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