Dan Menasco (Presenter)
Biotage
Authorship: Victor Vandell±, Dan Menasco±, Elena Gairloch±, Lee Williams¥ Rhys Jones¥, Paul Roberts¥
Biotage- Charlotte, NC
| Short Abstract Introduction: Screening urine samples quickly for drugs is typically performed by immunoassay analyzers. Once sample test positive for drug presence then an additional step is needed to qualitatively and quantitatively confirm the identity and amount of drug identified from the initial screening. Ideally, the workflow of a laboratory could be optimized by conducting the screening and confirmation analysis via tandem LC-MS simultaneously. To accomplish this, a reliable sample preparation method is needed to extract acidic, basic and neutral drugs from urine. This poster will outline the details for a novel sample preparation method can be used to extract acidic, basic and neutral drugs from hydrolysed urine samples. Over 32 different drugs were extracted from urine samples quickly and analysed qualitatively and quantitatively. |
Long Abstract
Introduction:
Screening urine samples quickly for drugs is typically performed by immunoassay analyzers. Once sample test positive for drug presence then an additional step is needed to qualitatively and quantitatively confirm the identity and amount of drug identified from the initial screening. Ideally, the workflow of a laboratory could be optimized by conducting the screening and confirmation analysis via tandem LC-MS simultaneously. To accomplish this, a reliable sample preparation method is needed to extract acidic, basic and neutral drugs from urine. This poster will outline the details for a novel sample preparation method can be used to extract acidic, basic and neutral drugs from hydrolysed urine samples. Over 32 different drugs were extracted from urine samples quickly and analysed qualitatively and quantitatively.
Aim:
This poster outlines a sample preparation method that extracts acidic, basic, and neutral drugs from urine using Supported Liquid Extraction.
Method:
Supported Liquid Extraction in a 96 fixed well plate format was used to extract 32 different drugs from urine. The drugs ranged in chemical polarity and hence would prove difficult to simultaneously extract using traditional SPE methods. The drug classes tested were opiates, barbiturates, benzodiazepines, tetrahydrocannabinoid and its metabolites and other pharmaceutical drugs. The drugs were spiked into urine and then subjected to enzymatic hydrolysis conditions. The drugs were then extracted from the hydrolysed urine using supported liquid extraction using a LOAD-WAIT-ELUTE format. The analytes were extracted at concentrations ranging from 1.25 - 100 ng/mL.
Results:
The drug analytes were recovered at three concentrations across the dynamic range of 25, 50 and 100 ng/mL. The recoveries ranged from 25-115%. All averaged recovery % RSDs were calculated at less than 10%. Limit of detection was determined to range from 1.25 – 5.0 ng/mL for all of the analytes. Measured matrix effects ranged from 20-25% for the analytes.
Conclusion:
We present a fast simplified approach for extraction of acidic, basic, and neutral drugs with reproducible recoveries for low analyte detection levels using a minimal amount of sample (100 µL).
References & Acknowledgements:
| Description | Y/N | Source |
| Grants | no | |
| Salary | yes | Biotage |
| Board Member | no | |
| Stock | no | |
| Expenses | yes |
IP Royalty: no
| Planning to mention or discuss specific products or technology of the company(ies) listed above: | yes |