Brittany Kassim (Presenter)
National Institute of Standards and Technology
Bio: Brittany is employed by the National Institute of Standard and Technology (NIST) in the inorganic laboratory located in the Hollings Marine Laboratory. Her primary work has been to develop mass spectrometric based methods for the quantification of iodine biomarkers including thyroid hormones, with the aim of certifying currently available Standard Reference Materials (SRM). This work evolved into the development of innovative SRMs to support the NIH-ODS initiatives on iodine health worldwide, and provide a control material for the delivery of traceability and calibration tools. In addition to Brittany’s primary role in method development on iodine biomarkers, she has been highly involved in the assignment of certified values for trace elements in environmental, clinical and biological materials using mass spectrometry in support of the SRM program.
Authorship: Brittany Kassim, Jessica Reiner and Stephen Long.
National Institute of Standards and Technology, 331 Fort Johnson Road, Charleston SC 29412
| Short Abstract The aim of this work was to develop higher order analytical methods and apply these to value assign target iodine status biomarkers, namely thyroid hormones, in applicable reference materials for the delivery of traceability and calibration tools. Method development for the quantification of thyroid hormones specifically T4 and T3 has been accomplished by way of selective elemental detection utilizing liquid chromatography (LC) coupled to an inductively coupled plasma mass spectrometer (ICP-MS). This higher order analytical method was applied to value assign target T3 and T4 hormones in currently available SRMs. |
Long Abstract
Iodine is a critical element for human health and plays a major role in basal metabolic rate, glucose metabolism, lipid breakdown as well as postnatal growth of neurotransmitters and bone development. Iodine deficiency affects approximately two billion people worldwide and is recognized as a significant public health problem. Deficiencies in iodine intake and physiological status are often discovered by the measurement of urinary iodide concentration or thyroid stimulating hormone (TSH), and in extreme cases by the formation of goiters. Diet is the essential source of iodine intake. Soils, especially in mountainous regions, that are iodine deficient result in many staple food sources that are deficient as well. Iodine supplementations, such as iodized salt, multivitamins and kelp are used globally to increase iodine intake.
In the body, iodine intake regulates the controlled synthesis of thyroid hormones from the thyroid gland, which if disrupted results in thyroid disease. Hypothyroidism and hyperthyroidism are loosely defined and difficult to diagnose, in part due to the current antibody-dependent, indirect hormone measurement methods that are prone to inaccuracies. However, thyroid disease is considered a global issue with an estimated 200 million individuals affected worldwide, with an estimated 20 million individuals in the United States alone. Thyroid hormones thyroxine (T4) and 3,3’,5-triiodo-L-thyronine (T3) are clinically important for disease diagnosis, treatment monitoring and preventative assessments, however the currently available immunoassay methods for clinical measurements yield variable results.
In order to validate traditional or routine methods of clinical biomarker quantification, a Standard Reference Material (SRM) is often used. Clinical analyses for the quantification of thyroid hormones are not SI traceable because there are no higher order reference methods in clinical use or a certified reference material. Such a control material would allow for the calibration of secondary standards and clinical assays to be normalized to a known SI traceable value.
The aim of this work was to develop higher order analytical methods and apply these to value assign target iodine status biomarkers, namely thyroid hormones, in applicable reference materials for the delivery of traceability and calibration tools. Method development for the quantification of thyroid hormones specifically T4 and T3 has been accomplished by way of selective elemental detection utilizing liquid chromatography (LC) coupled to an inductively coupled plasma mass spectrometer (ICP-MS). This higher order analytical method was applied to value assign target T3 and T4 hormones in currently available SRMs.
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