Jae-Seok Kim (Presenter)
Halym University
Authorship: Lee EG(1), Choi EK(3), Shin S(2), Kim TS(2), Park KH(3), Kim YS(3), Kim JS(1)
(1) Department of Laboratory Medicine, Hallym University College of Medicine, Chuncheon, Korea.
| Short Abstract NTM species identification is important for the appropriate treatment of corresponding type and its clinical significance. In this study, 63 species of mycobacteria reference strains and 105 clinical isolates, identified by multi-locus sequencing, were analyzed by MALDI-TOF MS (Tinkerbell LT, ASTA, Suwon, Korea). We acquired specific signals of each individual mycobacterial species cultured on 3% ogawa and MGIT on the basis of unique mass fingerprints. In addition, several mass peaks were selected as diagnostic markers for species confirmation. In conclusion, Tinkerbell LT-MS could be applied for rapid and reliable identification of mycobacteria in clinical laboratory. |
Long Abstract
Introduction:
MALDI-TOF MS has been widely used as a rapid and accurate identification of bacteria. NTM identification at the species level is important for the appropriate treatment of corresponding type and its clinical significance.
Methods: In this study, we used 168 NTM isolates, including 63 species reference strains and 105 clinical isolates, previously identified by multilocus sequence typing. The positive isolates cultured on 3% Ogawa and MGIT, were extracted through heat inactivation and mechanic disruption with chemical solvents. For species-level discrimination, these isolates were analyzed by MALDI-TOF MS ( Tinkerbell LT, ASTA, Suwon, Korea ).
Results: We analyzed 168 NTM isolates comprising 63 separate mycobacterial species taken from positive solid and broth cultures. Tinkerbell LT was generated acceptable data of each individual mycobacterial species on the basis of unique mass fingerprints and peaks information. We compared the results of clinical isolates of the species and its corresponding reference strain as one another. In addition, several peaks were selected as diagnostic markers for species confirmation.
Conclusions:
In conclusion, Tinkerbell LT could be applied for rapid and reliable identification of mycobacteria in clinical laboratory.
References & Acknowledgements:
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