Arnie Aistars (Presenter)
Waters Corporation
Authorship: (1)Arnie Aistars, (2)Jonathan Danaceau, (3)Jonathan Turner
(1,2,3) Waters Corporation, Milford Massachusetts, USA
| Short Abstract High-performance liquid chromatography-tandem mass spectrometry (LC/MS/MS) has become a powerful tool for quantitative analysis of drugs of abuse in the field of forensic toxicology. While many reversed-phase columns have been used for these applications, columns that contain a phenyl functionality have shown to have unique selectivity for many drugs of abuse due to pi-pi bond interactions between the aromatic rings contained within the target analytes and the stationary phase. This poster highlights a novel, high efficiency 1.6 µm solid-core column for the separation of opiods, benzodiazepines, amines and PCP. |
Long Abstract
High-performance liquid chromatography-tandem mass spectrometry (LC/MS/MS) has become a powerful tool for quantitative analysis of drugs of abuse in the field of forensic toxicology. Many laboratories are transitioning to LC/MS/MS as they replace existing immunoassay and GC/MS based screening methods. Often LC/MS/MS is employed to update older LC/MS methods and consolidate multiple analyses into single panels. While many reversed-phase columns have been used for these applications, columns that contain a phenyl functionality have shown to have unique selectivity for many drugs of abuse due to pi-pi bond interactions between the aromatic rings contained within the target analytes and the stationary phase. This poster highlights a novel, high efficiency 1.6 µm solid-core column for the separation of opiods, benzodiazepines, amines and PCP. Shown will be increased throughput from this phenyl solid-core column, as well as the advantages of using a protic solvent vs. a non-protic solvent. Results show the benefits of using CORTECS UPLC Phenyl 1.6 µm columns. All peaks were highly efficient and all compounds with the potential to interfere with each other were baseline separated. The use of different organic mobile phases revealed significant selectivity differences that can be exploited, if necessary, to optimize separation of critical analyte pairs. This column represents the continued development of Waters’ CORTECS solid-core column family.
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