Hassan Alamri (Presenter)
Cleveland State University
Authorship: Hassan Alamri, M.S.1,2, Bruce Levison, Ph.D.1, Tomas Cajka, Ph.D.3, Oliver Fiehn, Ph.D.3, Stanley L. Hazen, M.D., Ph.D.1, Valentin Gogonea, Ph.D.1, 2*
(1) Departments of Cellular & Molecular Medicine, Cleveland Clinic, (2) Department of Chemistry, Cleveland State University, (3) Department of Molecular and Cellular Biology & Genome Center, UC Davis
| Short Abstract To identify novel biomarker for early identification of individuals at risk for future cardiovascular events, we performed non-targeted Gas Chromatography/Mass spectrometry-based (GC-MS) analysis on 1000 subjects at risk for CVD in a semi-quantitative fashion to screen for plasma small-molecule metabolites that predict risk for CVD. Among these metabolites, several mono-, di-, and tri-carboxylic acids presented a significant prediction for CVD risk. We are further developing a targeted Stable-isotope dilution high performance liquid chromatography-electrospray ionization-tandem mass spectrometry method (LC-ESI-MS/MS) for the quantitation of carboxylic acids and amino acids, in order to demonstrate their clinical utility by analyzing them in a cohort study of 2000 subjects. |
Long Abstract
Cardiovascular disease (CVD) is the leading cause of death worldwide. A novel biomarker for early identification of individuals at risk for future cardiovascular events is still critical to provide new ways to diagnose disease states and reveal new metabolic pathways connected to CVD pathogenesis. To identify novel biomarker, we performed non-targeted Gas Chromatography/Mass spectrometry-based (GC-MS) analysis on 1000 subjects at risk for CVD in a semi-quantitative fashion to screen for plasma small-molecule metabolites that predict risk for CVD. Among these metabolites, several mono-, di-, and tri-carboxylic acids presented a significant prediction for CVD risk. We are further developing a targeted Stable-isotope dilution high performance liquid chromatography-electrospray ionization-tandem mass spectrometry method (LC-ESI-MS/MS) for the quantitation of carboxylic acids and amino acids, in order to demonstrate their clinical utility by analyzing them in a cohort study of 2000 subjects.
References & Acknowledgements:
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| Salary | no | |
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| Expenses | no |
IP Royalty: no
| Planning to mention or discuss specific products or technology of the company(ies) listed above: | yes |