Yu-Chen Chang (Presenter)
California Department of Public Health
Bio: Research Scientist at California Department of Public Health Environmental Health Laboratory
Authorship: Yu-Chen Chang1, Su Zhang2, Yufeng Guan3, Wei Zhou1, Jianwen She1
1California Department of Public Health, Environmental Health Laboratory; 2Shanghai Institute of Food and Drug Control; 3School of Chemistry and Environment, South China Normal University
| Short Abstract A sensitive method for testing urinary concentrations of benzophenone-3 (BP-3) developed by our laboratory is routinely used in biomonitoring studies. In this presentation, we describe a new strategy to expand the testing panel to the family of structurally-related compounds using BP-3 as a model system. BP-3 and its analogs are used in personal care products, such as sunscreens, as well as food packaging, etc., to protect the skin and products from UV light. As some of the these chemicals have carcinogenic, endocrine disrupting, or developmental or reproductive toxicity, wide exposure to vulnerable populations, particularly women and infants, present potential health concerns. |
Long Abstract
A sensitive method for testing urinary concentrations of benzophenone-3 (BP-3) developed by our laboratory is routinely used in biomonitoring studies. In this presentation, we describe a new strategy to expand the testing panel to the family of structurally-related compounds using BP-3 as a model system. BP-3 and its analogs are used in personal care products, such as sunscreens, as well as food packaging, etc., to protect the skin and products from UV light. As some of the these chemicals have carcinogenic, endocrine disrupting, or developmental or reproductive toxicity, wide exposure to vulnerable populations, particularly women and infants, present potential health concerns.
We have developed analytical methods using Q Exactive Plus Orbitrap LC-MS/MS system for the present study. Briefly, urine samples were enzymatically digested with de-conjugation enzymes followed by solid-phase extraction (SPE). The SPE eluent was then chromatographically separated on a C18 column under gradient elution of acetonitrile and water containing 0.1% formic acid as the modifier. Mass spectrometer data acquisition was carried out using Xcalibur under Data-Dependent Acquisition mode with inclusion list specifically targeted for BP-3 structurally-related compounds. Full MS scan was from 100-700 m/z with resolution of 70k. Data-Dependent Acquisition was set to have the top 5 most intense ions per full scan fragmented with 2 m/z isolation window at resolution of 17.5k. The mass spectrometry data were further analyzed using software TraceFinder and Compound Discoverer against in-house compound database and spectra library. Detection of several BP-3 structurally-related compounds and urinary metabolites in human urine samples will be presented.
References & Acknowledgements:
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IP Royalty: no
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