Yeo-Min Yun (Presenter)
Konkuk University School of Medicine
Authorship: Yeo-Min Yun1, Hyun Jeong Kim1, Hee-Won Moon1, Mina Hur1, Junghan Song2
1Departments of Laboratory Medicine, Konkuk University School of Medicine, Seoul, Korea; 2Department of Laboratory Medicine, Seoul National University Bundang Hospital, Seongnam, Korea
| Short Abstract Serum samples of 160 healthy controls, 50 pregnant women, and 50 intensive care unit (ICU) patients were collected. Total 25(OH)D levels of each sample were measured by LC-MS/MS and three automated assays, including ADVIA Centaur (Siemens, Germany), Elecsys (Roche, Switzerland), and Architect i200SR (Abbott, US).The vitamin D results of all three routine immunoassays showed poor concordance compared with those of LC-MS/MS in ICU patients than those in healthy controls. In pregnant women, the vitamin D results of ADVIA Centaur showed the high positive bias compared with vitamin D values measured by LC-MS/MS. In conclusion, standardization of each immunoassays is essential for clinical use to estimate vitamin D status. Moreover, for the exact vitamin D status evaluation in ICU patients and pregnant women, using LC-MS/MS measurement would be recommended. |
Long Abstract
Introduction: The associations of vitamin D and various clinical conditions have led to an increased importance in 25-hydroxyvitamin D [25(OH)D] assay. We can use several automated 25(OH)D assays including liquid chromatography/tandem mass spectrometry (LC-MS/MS) and immunoassay. We aimed to evaluate accuracy of three routine vitamin D immunoassays compared with the reference measurement using LC-MS/MS in pregnant women and intensive care unit (ICU) patients.
Methods: Serum samples of 160 healthy controls, 50 pregnant women, and 50 intensive care unit (ICU) patients were collected. Total 25(OH)D levels of each sample were measured by LC-MS/MS and three automated assays, including ADVIA Centaur (Siemens, Germany), Elecsys (Roche, Switzerland), and Architect i200SR (Abbott, US).
Results: In healthy control group (n=160), the mean absolute bias [(routine immunoassay result) - (LC-MS/MS results)] were -5.84 ng/mL (95% confidence interval [CI], -14.70 to 3.01 ng/mL) in ADVIA Centaur, 9.25 ng/mL (95% CI, -6.29 to 24.80 ng/mL) in Elecsys, and 3.51 ng/mL (95% CI, -10.64 to 17.67 ng/mL) in Architect. In ICU patient group (n=50), the mean absolute bias were 8.76 ng/mL (95% CI, -14.70 to 32.21 ng/mL) in ADVIA Centaur, ng/mL -7.17 mL (95% CI, -27.91 to 13.56 ng/mL) in Elecsys, and -6.37 ng/mL (95% CI, -27.43 to 14.69 ng/mL) in Architect. In Pregnant women (n=50), the mean absolute bias were 16.66 ng/mL (95% CI, 4.38 ~ 28.93 ng/mL) in ADVIA Centaur, 0.72 ng/mL (95% CI, -5.18 ~ 6.63 ng/mL) in Elecsys, and 1.54 ng/mL (95% CI, -4.50 ~ 7.57 ng/mL) in Architect.
Conclusions: The vitamin D results of all three routine immunoassays showed poor concordance compared with those of LC-MS/MS in ICU patients than those in healthy controls. In pregnant women, the vitamin D results of ADVIA Centaur showed the high positive bias compared with vitamin D values measured by LC-MS/MS. In conclusion, standardization of each immunoassays is essential for clinical use to estimate vitamin D status. Moreover, for the exact vitamin D status evaluation in ICU patients and pregnant women, using LC-MS/MS measurement would be recommended.
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