MSACL 2016 US Abstract

3D Cartography of the Microbiome of the Human Skin and Lungs in Cystic Fibrosis

Pieter Dorrestein (Presenter)
UCSD

Bio: Dr. Dorrestein is Professor at the University of California - San Diego. He is the Director of the Collaborative Mass Spectrometry Innovation Center and a Co-Director, Institute for Metabolomics Medicine in the Skaggs School of Pharmacy & Pharmaceutical Sciences, Departments of Pharmacology, Chemistry and Biochemistry. Since his arrival to UCSD in 2006, Dr. Dorrestein has been pioneering the development of mass spectrometry methods to study the chemical ecological crosstalk between population of organisms for agricultural, diagnostic and therapeutic applications.

Short Abstract The microbiome is critical to human health yet we know little about the chemical environment that our microbes live in. In this presentation we will explore untargeted metabolomics strategies to reveal the chemical environment of the microbiome. First we will cover the 3D skin metabolome and how the skin metabolome is influenced by personal care, medications, diet and the microbiome. We will explore the chemical similarities and contrasts associated with the chemistry from the skin of people who do not use medications or personal care products. Secondly we will highlight the metabolome of a human lung associated with Cystic Fibrosis in 3D. Finally the presentation will present a case study of a CF patient that clinically cultured Pseudomonas but that ultimately passed due to an E.coli infection.

Long Abstract

The microbiome is critical to human health yet we know little about the chemical environment that our microbes live in. In this presentation we will explore untargeted metabolomics strategies to reveal the chemical environment of the microbiome. First we will cover the 3D skin metabolome and how the skin metabolome is influenced by personal care, medications, diet and the microbiome. We will explore the chemical similarities and contrasts associated with the chemistry from the skin of people who do not use medications or personal care products. Secondly we will highlight the metabolome of a human lung associated with Cystic Fibrosis in 3D. Finally the presentation will present a case study of a CF patient that clinically cultured Pseudomonas but that ultimately passed due to an E.coli infection. The mechanism of death is reported in the microbial inventory, transcriptomics as well as the metabolomics data and is the catalyst that is leading to the development a rapid response precision microbiome unit.

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