Richard Yost (Presenter)
University of Florida
Bio: Dr. Yost is the Colonel Allen and Margaret Crow Professor and Head of Analytical Chemistry at the University of Florida, and the Director of the NIH Southeast Center for Integrated Metabolomics. He is also a Professor of Pathology, Immunology, and Laboratory Medicine at UF and a Professor of Pathology at the University of Utah. Dr. Yost, recognized internationally as a leader in the field of analytical chemistry and mass spectrometry, may be best known for inventing the triple quadrupole mass spectrometer. The primary thrust of his group's current research is the development and application of innovative mass spectrometric methodologies for global and targeted metabolomics, lipidomics, clinical analysis, and biomarker detection.
Authorship: Richard A. Yost
University of Florida, Gainesville, FL
| Short Abstract Mass spectrometric imaging (MSI) provides a level of chemical information unmatched by any other imaging modality (including histopathology, MRI, and PET scans). Furthermore, MSI offers the potential for rapid and direct analysis of tissue even when an image is not of interest. This presentation will explore innovations in MSI and direct tissue analysis, focusing on new sampling methods (including real-time in situ microextraction using the flowprobe and desorption electrospray or DESI) and strategies for increasing the speed, spatial resolution, information content, and quantitative performance of the methods. Applications to be discussed will include characterization and biomarker detection in melanoma, pansteatitis, aging muscle, and Parkinson’s disease. |
Long Abstract
Mass spectrometric imaging (MSI) has evolved over the past decade into an invaluable technique for characterizing biological tissue and localizing metabolites, lipids, peptides and proteins. MSI takes advantage of the remarkable sensitivity and selectivity of mass spectrometry (including high revolution MS and tandem mass spec (MS/MS and MSn) to provide a level of chemical information unmatched by any other imaging modality (including histopathology, MRI, and PET scans). Furthermore, MSI can yield insight into hundreds of analytes in a single analysis, without labeling. Even when a chemical “image” is not of interest, MSI techniques can provide rapid and direct analysis of tissue, including samples too small for classic GC/MS or LC/MS analysis.
This presentation will explore innovations in MSI and direct tissue analysis, focusing on new sampling methods (including real-time in situ microextraction using the flowprobe and desorption electrospray or DESI) and strategies for increasing the speed, spatial resolution, information content, and quantitative performance of the methods.
This presentation will present data for a variety of applications, focusing on characterization and biomarker detection in a variety of diseased states. These studies will include the potential for rapid screening for skin cancer (melanoma), metabolomic studies of poorly characterized diseases such as pansteatitis and the effects of aging on muscle, and investigation of treatment modalities in diseases such as Parkinson’s.
References & Acknowledgements:
The authors acknowledge the support of NIH (Southeast Center for Integrated Metabolomics, U24 DK097209.
| Description | Y/N | Source |
| Grants | yes | Agilent Technologies, Prosolia, Wellspring, Breathtec |
| Salary | no | |
| Board Member | no | |
| Stock | yes | Breathtec |
| Expenses | no |
IP Royalty: no
| Planning to mention or discuss specific products or technology of the company(ies) listed above: | yes |