Vladimir Frankevich (Presenter)
Research Center for Obstetrics and Gynecology
Bio: Vladimir Frankevich is a head of System Biology Department at Federal State Budget Institution "Research Center for Obstetrics, Gynecology and Perinatology", Ministry of Healthcare of the Russian Federation. The work experience includes more than 10 years senior research position at Swiss Federal Institute of Technology (ETH, Zurich)
Authorship: Vladimir Frankevich, Vitaliy Chagovets, Alisa Tokareva, Alexey Kononikhin, and Gennady Sukhikh.
Federal State Budget Institution "Research Center for Obstetrics, Gynecology and Perinatology" Ministry of Healthcare of the Russian Federation
Chemical scanning of cancer tissues by the combination of mass spectrometry with molecular resonance probe desorption. Molecular composition differences in real time were shown for different cancer tissues. Multivariate analysis was used to extract useful information from mass spectrometric data. Score plots from OPLS-DA revealed good clasterization of data points with accordance to tissue source. Real-time feedback to the surgeon about margin status in patients with breast cancer was shown. High accuracy tumor boundary detection is also presented.
Quantum Molecular Resonance (QMR) technology was recently developed as a surgical tool for tissue dissection . In QMR a well-defined spectrum of high-frequency electromagnetic field is produced to selectively break molecular bonds without inducing a significant rise in local temperature. In general purposes QMR was designed for the surgery. Here we demonstrate QMR technology in combination with MS as a novel chemical scanning technique. We show that this technique allows on-line ambient molecular analysis of biological tissues with high chemical sensitivity.
The Vesalius (Telea, Italy) quantum molecular resonance generator was used as a desorption probe for biological tissues chemical scanning. The probe was coupled with air jet pump which delivered desorbed molecules into the home-made EESI source coupled with qTOF mass spectrometer (Maxis, Bruker Daltonics, Germany). Tissue samples were obtained in the Research Center for Obstetrics, Gynecology and Perinatology and stored under 193 K until measurements. All data were processed with PCA and OPLS-DA methods to determine the most significant differences between tissues.
The method utilized in the study allowed obtaining highly intensive, stable and reproducible signal from tissues in both positive and negative ion modes. Molecular composition differences in real time were shown for different cancer tissues. Multivariate analysis was used to classify the mass spectrometric data. Score plots from OPLS-DA revealed good clasterization of data points with accordance to tissue source. Real-time feedback to the surgeon about margin status in patients with breast cancer was shown. High accuracy tumor boundary detection is also presented.
Conclusions & Discussion
A novel on-line ambient platform for biological tissue analysis is introduced
References & Acknowledgements:
1. Pozzato G, Vignato G: Teoria della risonanza quantica molecolare nella realizzazione del bisturi elettronico ‘Vesalius’. Quintessence Int 2003;5/6:153–155.
2. Chen, H., A. Venter, and R.G. Cooks, Extractive electrospray ionization for direct analysis of undiluted urine, milk and other complex mixtures without sample preparation. Chemical Communications, 2006(19): p. 2042-2044.
This work was supported by Russian Science Foundation grant (agreement No. 16-14-00029).
IP Royalty: no
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