MSACL 2017 EU Abstract

Time-Course Analysis of 3-epi-25-Hydroxyvitamin D3 Shows Markedly Elevated Levels in Early Life, Particularly from Vitamin D Supplementation in Preterm Infants

Jody van den Ouweland (Presenter)
Canisius-Wilhelmina Hospital

Authorship: Nina Ooms1, Henny van Daal2, Antonius M. Beijers2, Peter Gerrits1, Ben Semmekrot1, Johannes M.W. van den Ouweland2
1Department of Pediatrics and 2Department of Clinical Chemistry, Canisius Wilhelmina Hospital, Nijmegen, The Netherlands

Short Abstract

We have studied dynamics of 3-epi-25(OH)D3 formation during infancy by measuring 25(OH)D3 and 3-epi-25(OH)D3 levels by LC-MS/MS in (early)preterm and term infants up to 2 years of age. At birth, all infants showed low contribution of 3-epi-25(OH)D3, increasing the week after starting vitamin D supplementation, until three months of age. Highest levels of 3-epi-25(OH)D3 were found in early preterm infants, supporting the hypothesis that hepatic immaturity plays a role in 3-epi-25(OH)D3 formation.

Long Abstract

Introduction

An epimeric form of 25-hydroxyvitamin D3 (25(OH)D3) has recently been detected in clinical samples, with relatively high levels in infants. Little is known on 3-epi-25(OH)D3 formation and physiological function. Our objective was to study dynamics of 3-epi-25(OH)D3 formation during infancy.

Methods

25(OH)D3 and 3-epi-25(OH)D3 levels were measured by LC-MS/MS in 22 preterm (aged 34-37 wks), 15 early preterm (<34 wks) and 118 term infants up to 2 years of age. All infants were prescribed vitamin D 400 IU/day after the first week of life.

Results

At birth, 3-epi-25(OH)D3 levels were 3 (1-7) nmol/L, <10% of total 25(OH)D3. From the second week to three months of age, both 25(OH)D3 and 3-epi-25(OH)D3 increased, with highest 3-epi-25(OH)D3 contribution in early preterm infants (up to 55% of total 25(OH)D3 versus 36% in term infants, p<0.0001). After three months of age, 3-epi-25(OH)D3 normalized to <10% in all infants.

Conclusions & Discussion

At birth, all infants showed low contribution of 3-epi-25(OH)D3, increasing the week after starting vitamin D supplementation, until three months of age. Highest levels of 3-epi-25(OH)D3 were found in early preterm infants, supporting the hypothesis that hepatic immaturity plays a role in 3-epi-25(OH)D3 formation.


References & Acknowledgements:

Ooms N, van Daal H, Beijers AM, Gerrits P, Semmekrot B, van den Ouweland JMW. Time-course analysis of 3-epi-25-hydroxyvitamin D3 shows markedly elevated levels in early life, particularly from vitamin D supplementation in preterm infants. Pediatr Res, 2016;79:647-53.


Financial Disclosure

DescriptionY/NSource
Grantsno
Salaryno
Board MemberyesSciComm MSACL
Stockno
Expensesno

IP Royalty: no

Planning to mention or discuss specific products or technology of the company(ies) listed above:

no