Jentaie Shiea (Presenter)
National Sun Yat-Sen University
Bio: Jentaie Shiea received his bachelor degree in Chemistry from National Chung-Hsing University, Taiwan in 1981. He received M.S. (1988) and Ph.D. (1991) degrees from Montana State University (MSU). His thesis studies focus on Organic Geochemistry and Analytical/Physical Chemistry. After post-doctoral fellowship at Pennsylvania State University (Department of Material Science), he joined National Sun Yat-Sen University (NSYSU, Kaohsiung, Taiwan) faculty. He is now a distinguish professor of Department of Chemistry at NSYSU. He authored or co-authored more than 150 scientific publications and presentations at internationally scientific conferences. He is the Fellow of Royal Society of Chemistry (FRSC) and is the editorial or advisory board member of Anal. Methods, Mass Spectrom., Int. J. Mass Spectrom., Mass Spectrom. Let., The Scientific World J., and Cur. Chromatogra.
Authorship: Jentaie Shiea
Department of Chemistry, National Sun Yat-Sen University, Kaohsiung, Taiwan
| Short Abstract Skin plays a vital role in protecting human being against the attack by pathogenic microorganisms. Metabolites are also released from skin through numerous glands underneath it. Although some metabolites on skin are potential disease biomarkers, current analytical techniques are incapable to efficiently identify them on skin. In this study, TD-ESI/MS, a novel ambient mass spectrometric technique, was used to characterize the skin compounds including drugs, lipids and metabolites without doing any sample pretreatment. Herein, ambient imaging was demonstrated for visualizing the distribution of drugs and metabolites on whole body skin. After medicine was taken by volunteer for a period of time, the distribution of medicines or their metabolites was collected and analyzed. |
Long Abstract
Skin plays a vital role in protecting human being against the attack by pathogenic microorganisms. Metabolites are also released from skin through numerous glands underneath it. Although some metabolites on skin are potential disease biomarkers, current analytical techniques are incapable to efficiently identify them on skin. In this study, thermal desorption electrospray ionization/mass spectrometry (TD-ESI/MS), a novel ambient mass spectrometric technique, was used to characterize the skin compounds including drugs, lipids and metabolites without doing any sample pretreatment. Since TD-ESI/MS owns the feature of rapidness, high-throughput sample analysis for skin imaging on specific chemical compounds can be performed. Herein, ambient imaging was demonstrated for visualizing the distribution of drugs and metabolites on whole body skin. After medicine was taken by volunteer for a period of time, the distribution of medicines or their metabolites was collected and analyzed. A stainless steel probe was used to scrap the skin surface for ca 1.5 cm for sampling. It was then inserted into a heated oven to thermally desorb the analytes on it. The analytes were carried by a nitrogen stream down to a plume consisting of acidic methanol/water and ionized by reacting with the charged species (e.g. H+, H3O+, etc) in the ESI plume. The remaining samples were rapidly removed by burning the probe with a torch. It took less than 30 seconds to complete an analysis. In typical case, more than 3000 samples were collected on skin within an hour and the analysis of these samples took more than 15 hours. The imagings were obtained using commercial available imaging software. Taking caffeine as an example, the analyte ion signal was mostly detected on the upper body especially forehead, fingertips and backside of the body. This may due to different sweating rates in different skin parts as well as the distribution of veins and capillaries over the body. Not only medicines and their metabolites but regular metabolites such as cholesterol, squalene, fatty acids, amino acids, diglycerides, and triglycerides were also detected and their whole body skin imagings show that the distribution of metabolite on skin also depend on the structure and chemical property of the compounds.
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