Grace van der Gugten (Presenter)
St. Paul’s Hospital
Authorship: Discussion Facilitators:
Jane Dickerson,
Deborah French,
Autumn Breaud,
Josh Akin,
Josh Hayden,
Julia Drees,
Sarah Shugarts
| Short Abstract When developing and validating an LC-MS/MS assay, matrix effects must be assessed. But what should be done when the post column infusion and/or phospholipid scans show undesirable results? And how do we correctly calculate spikes for the quantitative matrix effects experiment? In this interactive session, attendees will work through a problem set to help better understand how to perform matrix effects experiments and interpret the results. |
Long Abstract
When developing and validating an LC-MS/MS assay, matrix effects must be assessed. But what should be done when the post column infusion and/or phospholipid scans show undesirable results? And how to we correctly calculate spikes for the quantitative matrix effects experiment?
In this interactive session, attendees will work in small groups through an example problem set to:
1) Perform the calculations needed for quantitative matrix effects experiment for a liquid-liquid extraction LC-MS/MS assay
2) Interpret results from a post column infusion to determine areas of ion suppression
3) Interpret results from a phospholipid MRM monitoring experiment
Concluding this session we will review the problem set from the podium.
Learning objectives:
1) Understand how to calculate the pre, post and non-matrix spikes for the quantitative matrix effects experiment.
2) Understand how to interpret chromatograms from post column infusion and phospholipid monitoring experiments.
3) Take away strategies for mitigating/dealing with matrix effects.
References & Acknowledgements:
| Description | Y/N | Source |
| Grants | no | |
| Salary | no | |
| Board Member | no | |
| Stock | no | |
| Expenses | no |
IP Royalty: no
| Planning to mention or discuss specific products or technology of the company(ies) listed above: | no |