Timothy Garrett (Presenter)
University of Florida
Authorship: Timothy J Garrett1, Emily L Gill2, Louis Searcy2, Richard A Yost2
University of Florida Departments of 1Pathology and 2Chemistry
| Short Abstract Liquid microjunction surface analysis (LMSA) is a direct tissue analysis sampling approach that enables the rapid sampling of a tissue surface for many applications such as metabolomics, imaging, and analysis of short lived chemical species. The application to clinical specimens offers a rapid approach to tissue analysis that could improve diagnosis and treatment. We report the use of LMSA on the analysis of melanoma as well as the application to detecting transient chemical signatures produced by deep brain stimulation. |
Long Abstract
Melanoma is an aggressive skin cancer that has a better prognosis when detected early; unfortunately, early recognition of melanoma and histological diagnosis has dificulties.1 Identification of biomarkers could improve the diagnosis of melanoma before metastasis develops. Many studies have explored biomarkers for cancer.1–3 Few studies have reported biomarker identifications for melanoma through exploration of the metabolome and lipidome of tissue. This work explores the lipid profiles of normal and melanoma skin tissues using liquid micro junction surface sampling probe – mass spectrometry (LMJSSP-MS), which allows for direct analysis of biopsied skin tissue.4 A variety of other cancers have been analyzed by other ambient ionization techniques, the most comparable being desorption electrospray ionization (DESI).5,6 Lipid identification used an in-house fragmentation library and commercially available software. The biochemical profiles of the putative biomarkers were further explored to better understand the pathology of melanoma.
Deep brain stimulation (DBS) is a surgical treatment for Parkinson’s disease, yet little is known regarding the mechanism of action or the chemicals produced during stimulation. LMJSSP coupled to MS offers a unique opportunity to probe for changes as a result of DBS. This work with LMJSSP explores the ability of the technique to detect transient chemical species in tissue.
References & Acknowledgements:
(1) Weinstein, D.; Leininger, J.; Hamby, C.; Safai, B. J. Clin. Aesthet. Dermatol. 2014, 7 (6), 13–24.
(2) Gogas, H.; Eggermont, A. M. M.; Hauschild, A.; Hersey, P.; Mohr, P.; Schadendorf, D.; Spatz, A.; Dummer, R. Ann. Oncol. 2009, 20 (SUPPL. 4), 8–13.
(3) Fernandis, A. Z.; Wenk, M. R. J. Chromatogr. B Anal. Technol. Biomed. Life Sci. 2009, 877 (26), 2830–2835.
(4) Van Berkel, G. J.; Kertesz, V.; King, R. C. Anal. Chem. 2009, 81 (16), 7096–7101.
(5) Abbassi-Ghadi, N.; Jones, E. A.; Gomez-Romero, M.; Golf, O.; Kumar, S.; Huang, J.; Kudo, H.; Goldin, R. D.; Hanna, G. B.; Takats, Z. J. Am. Soc. Mass Spectrom. 2015, 7, 71.
(6) Guenther, S.; Muirhead, L. J.; Speller, A. V. M.; Golf, O.; Strittmatter, N.; Ramakrishnan, R.; Goldin, R. D.; Jones, E.; Veselkov, K.; Nicholson, J.; Darzi, A.; Takats, Z. Cancer Res. 2015, 75 (9), 1828–1837.
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