Livia Schiavinato Eberlin (Presenter)
The University of Texas at Austin
Authorship: Livia Schiavinato Eberlin
The University of Texas at Austin
| Short Abstract Ambient ionization MS provides great analytical sensitivity, specificity, and depth of molecular information in real time, directly from tissue samples, thus, the prospect of using this technology in and outside of the operating room is broad and exciting. Yet, is it realistic to expect ambient ionization MS to become an integral clinical technology for biopsy analysis? In this talk, examples of research progress being undertaken towards this goal using ambient ionization MS will be presented, including 1. Pre-operative diagnosis of fine needle aspirate biopsy samples, 2. Intra-operative diagnosis of touch imprint biopsies. Our results suggest that ambient ionization mass spectrometry can be successfully used for rapid cancer diagnosis from small biopsy samples in a profiling mode. |
Long Abstract
Biopsy evaluation is the standard of care for preoperative screening and diagnosis of many solid tumors. Different biopsy techniques are used to obtain small tissue samples. Fine-needle aspiration (FNA) procedures represent rapid, efficient, and minimally invasive means to sample superficial and deep lesions. For example, FNA is well established in the primary diagnosis of thyroid and lung cancers, and is often used to determine cancer subtype and molecular information to tailor specific cancer therapies. FNA material is typically expelled onto a slide to prepare smears which are subjected to standard histological procedures for immediate evaluation by a cytopathologist. Nevertheless, the ability to provide morphological distinction between tumor types from biopsy material for certain tumor types can be difficult even for skilled pathologists. Similarly, rapid intra-operative techniques include touch imprint cytology, which is commonly used for sentinel lymph node (SLN) in order to provide staging information for a variety of cancers. Touch imprint (TI) cytology can be performed rapidly, yet, at a low sensitivity. Here, we investigated the utility of ambient ionization mass spectrometry for rapid diagnosis of FNA and TI samples. Human tissue samples including cancer and normal tissues were obtained. FNA and TI biopsies were performed in the laboratory mimicking standard procedures performed in clinic. Samples were analyzed using a commercial desorption electrospray ionization mass spectrometry imaging (DESI-MSI) system (Prosolia Inc., Indianapolis, IN) coupled to an Orbitrap mass spectrometer (Thermo Fisher Scientific, San Jose, CA). DESI-MSI was performed in the negative ion mode from m/z 100-1500 using the orbitrap as the mass analyzer. 200 μm of spatial resolution was set for the imaging experiments. After DESI-MSI, the same samples were stained and diagnosed by histopathology. Raw data was imported to R language for statistical analysis using the Lasso method. Statistical classifiers were built to recognize different classes of tissues. The results obtained demonstrate that DESI-MS can be used to to rapidly diagnose several cancer types from biopsy samples with good sensitivity and accuracy.
References & Acknowledgements:
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| Grants | no | |
| Salary | no | |
| Board Member | yes | |
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| Expenses | no |
IP Royalty: no
| Planning to mention or discuss specific products or technology of the company(ies) listed above: | no |