Garry Nolan (Presenter)
Stanford University School of Medicine
High parameter single cell analysis has driven deep understanding of immune processes. Using a next-generation single-cell “mass cytometry” platform we quantify surface and cytokine or drug responsive indices of kinase target with 45 or more parameter analysis (e.g. 45 antibodies, viability, nucleic acid content, and relative cell size). Similarly, we have developed two advanced technologies that enable deep phenotyping of solid tissue in both fresh frozen and FFPE formats (50 – 100 markers). We have recently extended this parameterization to mRNA with the capability to measure down to 5 molecules per cell in combination with any other set of previously created markers.
References & Acknowledgements:
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