Autumn Breaud (Presenter)
Johns Hopkins University
Authorship: Autumn Breaud, William Clarke, PhD
Johns Hopkins University
| Short Abstract When developing HPLC-MS/MS methods for therapeutic drug monitoring or toxicology, it is important to work with a sample matrix which closely matches the matrix of unknown samples. Generally, this involves the use of pooled materials for which no demographic information is available. In this session, we discuss the discovery of age-specific matrix interference during the development of an assay for quantification of chlorhexidine, an antiseptic agent used for washing neonatal patients. |
Long Abstract
When developing HPLC-MS/MS methods for therapeutic drug monitoring or toxicology, it is important to work with a sample matrix which closely matches the matrix of unknown samples. Generally, this involves the use of pooled materials for which no demographic information is available. In this session, we discuss the discovery of age-specific matrix interference during the development of an assay for quantification of chlorhexidine, an antiseptic agent used for washing neonatal patients. We will discuss 1) the impact of using non-age-matched matrix to calibrate this assay, and 2) a technique for managing the requirement for age-matched standards, and 3) considerations for development of other HPLC-MS/MS assays.
References & Acknowledgements:
| Description | Y/N | Source |
| Grants | yes | Thermo Scientific |
| Salary | no | |
| Board Member | no | |
| Stock | no | |
| Expenses | no |
IP Royalty: no
| Planning to mention or discuss specific products or technology of the company(ies) listed above: | yes |