MSACL 2018 US Abstract

Topic: Practical Training

A Brief Introduction to the Basics of LC-MS in the Clinical Laboratory

Nandu Chindarkar (Presenter)
Kaiser Permanente Regional Lab

Bio: Nandu Chindarkar, PhD, is a Technical Director (Clinical Chemistry) at the Kaiser Permanente Regional Laboratory, Berkeley, CA.

Authorship: Nandu Chindarkar, PhD
Kaiser Permanente Regional Lab, Berkeley, CA

Short Abstract

Bio: Nandu Chindarkar, PhD, is a Technical Director (Clinical Chemistry) at the Kaiser Permanente Regional Laboratory, Berkeley, CA. Dr. Chindarkar has worked on various mass spectrometry platforms over the last 14 years. He has extensive experience in developing LC-MS methods for clinical/toxicology applications. Short Abstract: The goal of this presentation is to introduce the audience to liquid LC-MS platform used in clinical laboratories. It provides a brief introduction to LC, ESI, and tandem quadrupole mass analyzer. Furthermore, an overview of detection and quantitation of an analyte using LC-MS will be provided.

Long Abstract

Introduction

Introduction of the electrospray ionization (ESI) technique in the late 1980s led to the successful hyphenation of two powerful analytical tools i.e. liquid chromatography (LC) and mass spectrometry (MS). Over the last two decades, LC-MS has become one of the essential analytical platforms in laboratory medicine. It plays a significant role in providing a high quality patient care and will continue to do so in future. Based on these trends, understanding of LC-MS is crucial to the success of a clinical laboratorian.

Methods

The goal of this presentation is to introduce the audience to liquid LC-MS platform used in clinical laboratories. It provides a brief introduction to LC, ESI, and tandem quadrupole mass analyzer. Furthermore, an overview of detection and quantitation of an analyte using LC-MS will be provided.

Results

The following topics will be covered;

- basic principles of LC, MS, and ESI

- brief discussion of tandem MS, collision induced dissociation (CID), and multiple reaction monitoring (MRM)

Conclusions & Discussion

After attending this session, the participants should be able to briefly describe:

ƒ{- the LC, MS, and ESI techniques

ƒ{- the tandem MS concept utilized in clinical/toxicology laboratory

ƒ{- separation and detection of analytes using LC-MS platform


References & Acknowledgements:

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Financial Disclosure

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IP Royalty: no

Planning to mention or discuss specific products or technology of the company(ies) listed above:

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