MSACL 2018 EU Abstract

Self-Classified Topic Area(s): Metabolomics

Use of Quantitative Metabolomics for Investigation of Age-Related Nuclear Cataracts

Vadim V. Yanshole (1,2), Lyudmila V. Yanshole (1,2), Olga A. Snytnikova (1,2), Kudryavtsev I.S. (3), Yuri P. Tsentalovich (1,2)
(1) International Tomography Center SB RAS, Novosibirsk, Russia (2) Novosibirsk State University, Novosibirsk, Russia (3) Novosibirsk Regional Clinical Bureau of Forensic Medical Examination, Novosibirsk, Russia

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Vadim Yanshole (Presenter)
International Tomography Center SB RAS

Presenter Bio: Vadim Yanshole obtained M.Sc. (Hons) at the Novosibirsk State University (Novosibirsk, Russia, 2001-2007). In 2011 he received Ph.D. in chemical physics.
Since 2007 he is working at the ITC SB RAS (Novosibirsk, Russia) in the fields of proteomics, metabolomics and mass-spectrometry; now he is the leading specialist in the institute in these fields. He is experienced in MALDI-TOF and high-resolution LC-MS techniques, and their use in the biological applications.
Current research interest is mainly devoted to quantitative metabolomic profiling of the different human tissues and liquids, especially eye constituents – lens, aqueous humor, and cornea. Recent investigations include the metabolomic analysis of lens of different animal species.

Relevant Financial Disclosures (within past 24 months)

Grant/Research Support Russian Foundation for Basic Research (RFBR)

Discussing Products? NO, presenter will NOT present educational content related to products/services of the ineligible company(ies) listed above.

Conflict mitigation NOT REQUIRED.

Abstract

A cataract (clouding of the lens) is the most common cause of vision declining of older people. Unlike most other human tissues, the lens has specific structure to be transparent: firstly, the lens consists mainly of fiber cells without organelles, and secondly, it lacks blood vessels. The protection of the lens is mainly provided by metabolites; most of them are synthesized in the lens epithelium or enter the lens through the epithelial layer from the surrounding aqueous humor (AH). Therefore, changes in the metabolome of the lens and AH may help to establish the molecular mechanisms of the cataract onset.
Current report provides the data analysis of changes in the metabolomic profiles of human eye lens and AH under the development of cataracts. The concentration of more than 80 metabolites in the lens and AH have been determined with the combined use of LC-MS, LC-OD and NMR methods.