MSACL 2018 EU Abstract

Self-Classified Topic Area(s): Microbiology

MALDI-TOF Mass Spectrometry: A Method for the Detection of Bacterial Contamination in Platelet Concentrates

Yasmine Chetouane* (1), Pierre Gallian (2), Jacques Chiaroni (3), Didier Raoult (1), Laurence Camoin-Jau (1)
1-Aix-Marseille University, IHU Mediterranean infection, Research Unit Microbes Evolution Phylogeny and Infections (MEPHI). 2- Etablissement Français du Sang (EFS) La Plaine Saint-Denis, France 3- Aix-Marseille University, CNRS, EFS, ADES UMR 7268, Marseille, France

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Yasmine Chetouane (Presenter)
IHU méditerranée infection

Presenter Bio: Yasmine, doctoral student in the 3rd year of the thesis.She works in the research unit on Microbes Evolution Phylogeny and Infections (MEPHI), on the Mediterranean infection site in Marseille. She is working on a new approach to detect bacterial contamination in platelet concentrates before transfusion in collaboration with the French Blood Establishment of Marseille, led by Prof. Jacques Chiaroni.

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Abstract

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been recently introduced in microbiological laboratories for the identification of microorganisms with proteomics approaches. Here, we investigate the use of MALDI-TOF MS by means of a Microflex MS (MALDI Biotyper-Bruker Daltonik, Germany) for the rapid, cheap and reliable detection and identification of bacterial contamination in Platelets Concentrates (PCs). We have demonstrated the stability and reproducibility of the spectrum over time and whatever the blood group of PCs. Also, we have compared the sensitivity and the specificity of the BACTEC cultivation method to the MALDI-TOF.