MSACL 2018 EU Abstract

Self-Classified Topic Area(s): Small Molecules

Simultaneous and Automated LC-MS Analysis of Plasma Methylmalonic Acid and Short Chain Fatty Acids Using a Carbodiimide-based Derivatization

M.S. Pratt, C.P. van der Ley, I. Wijbenga-van der Kooi, W.H.A. de Jong, M. van Faassen, I.P. Kema
Department of Laboratory Medicine, University Medical Center Groningen, University of Groningen, The Netherlands

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Mark Pratt (Presenter)
University Medical Center Groningen

Presenter Bio: I am a PhD-student at the department of Laboratory Medicine of the University Medical Centre Groningen in the lab of prof. Ido Kema. The laboratory is specialized in the LC-MS/MS analysis of low molecular weight neuroendocrine biomarkers. My focus is on the development of new strategies for the routine analysis of challenging (combinations of) small molecules, as well as neuroendocrine peptides and proteins.

Relevant Financial Disclosures (within past 24 months)

Salary Spark Holland (50% sandwich PhD-student)

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Abstract

Methylmalonic acid has long been regarded as the most sensitive and specific functional marker of vitamin B12 status. However, it may not tell the complete story. Bacteria in the small intestine use vitamin B12 as a cofactor for the metabolism of succinic acid to propionic acid. Therefore, if vitamin B12 is utilized by bacterial overgrowth in the ileum, a vitamin B12 deficiency may occur despite sufficient dietary intake. Furthermore, this may result in high concentrations of the cytotoxic propionic acid.
We have developed a novel LC-MS method for the simultaneous quantification of methylmalonic acid, propionic acid, succinic acid and butyric acid using a carbodiimide-based derivatization. This method may provide increased insight into the cause of vitamin B12 deficiencies, allowing for improved treatment strategies.