A Newly Developed LC-MS/MS Method for the Quantitation of Glucagon in Plasma Shows the Lack of Specificity of Former RIA Methods
Jordi Farré-Segura(1), Neus Fabregat-Cabello(1), Laurent Nyssen(1), Stéphanie Peeters(1), Caroline Le Goff(1), Etienne Cavalier(1). (1)Department of Clinical Chemistry, University Hospital of Liège, University of Liège, Belgium.
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Jordi Farre-Segura (Presenter) University of Liège, CHU de Liège
Presenter Bio: Jordi Farré obtained his degree and master-equivalent diploma in pharmacy in September 2017 by University of Barcelona. In 2016, he was firstly introduced to the clinical chemistry field in his hometown hospital’s laboratory (Althaia-Xarxa Hospitalària Universitària. Manresa, Spain) as a trainee. Later this same year he was granted with an Erasmus exchange to Liège, where he develops his research in proteomics since October 2017 as a PhD student at the Department of Clinical Chemistry, University Hospital of Liège (Belgium) under the supervision of Prof. Etienne Cavalier and Dr. Anne-Catherine Servais.
During his first stay in Belgium he contributed in the development of a new LC-MS/MS method for quantitation of Vitamin D metabolites in serum, under the supervision of Dr. Neus Fabregat and Dr. Caroline Le Goff.
Relevant Financial Disclosures
(within past 24 months, reported on Apr 06, 2021)
No relevant financial relationship(s) to disclose.
Abstract
We have developed a sensitive method for intact glucagon quantitation by LC-MS/MS based on a simple solid phase extraction. The method showed good precision, accuracy and detection limits throughout its advancement. However, our preliminary comparison with our routine radioimmunoassay (RIA) showed a significant bias. Hence, RIA provides spurious results compared to the LC-MS/MS that could suppose a change in the perspective of this peptide’s measurement.
= Discovery stage.
= Translation stage.
= Clinically available.
