MSACL 2018 EU Abstract

Self-Classified Topic Area(s): Microbiology

In-situ Detection of the Botulinum Neurotoxin A by Functionalized MALDI Chips

Petra Darebna(1,2), Petr Pompach (1,2), Jiri Dresler (3), Petr Novak (1,2)
(1) Institute of Microbiology, v.v.i., Czech Academy of Sciences, CZ (2) Faculty of Science, Charles University in Prague, CZ (3) Department of Microbiology and Biological Defence research, Military Health Institute, CZ

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Petra Darebna (Presenter)
BioCeV

Relevant Financial Disclosures (within past 24 months, reported on Sep 20, 2021)

Grant/Research Support Grant Agency of Charles University

Salary Czech Academy of Sciences

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Abstract

Botulinum neurotoxin (BoNT) is well known for causing the lethal disease botulism. The only widely accepted test for the identification of BoNTs in both clinical specimens and food is the mouse bioassay. In this study, we use MALDI chips functionalized by biotin-binding protein neutravidin prepared by ambient soft ion landing at atmospheric pressure for enrichment and detection of biotinylated peptides – products of specific endoproteinase activity of BoNT/A. The functionality of surfaces modified with biotin-binding molecules could help to drive the MALDI-TOF mass spectrometry toward its implementation into routine clinical practice.