MSACL 2018 EU Abstract

Self-Classified Topic Area(s): Glycomics

In-Depth Characterisation of Heavily Glycosylated Proteins and Their Potential as Colorectal Cancer Biomarkers

Valeriia Kuzyk (1,2), Guinevere S.M. Kammeijer (2), Rob Haselberg (1), Manfred Wuhrer (2), Govert W. Somsen (1)
(1) Vrije Universiteit Amsterdam, Division of Bioanalytical Chemistry, Amsterdam, The Netherlands, (2) Leiden University Medical Center, Center for Proteomics and Metabolomics, Leiden, The Netherlands

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Valeriia Kuzyk (Presenter)
Vrije Universiteit Amsterdam

Presenter Bio: Completed Bachelors degree in Biology and Master degrees in Biochemistry and Bioinformatics. Research interests: glycomics, proteomics, mass-spectrometry, capillary electrophoresis, biomarker research, method development.

Relevant Financial Disclosures (within past 24 months, reported on Jan 14, 2022)

Grant/Research Support European Commission, Horizon 2020 programme under grant agreement number 676421

Discussing Products? NO, presenter will NOT present educational content related to products/services of the ineligible company(ies) listed above.

Conflict mitigation NOT REQUIRED.

Abstract

Protein glycosylation is known to rapidly change in disease, as well as in cancer progression. Therefore, heavily glycosylated tumor-derived circulating proteins hold a great potential to perform as “disease ambassadors” in the bloodstream, enabling more sensitive and specific diagnostics. However, proper characterization is challenging due to low quantity in serum and the high heterogeneity of the glycan distribution. Here, we present a comparison between different analytical platforms for the in-depth characterization of two glycoproteins (carcinoembryonic antigen and laminin B1). Further studies will examine if altered glycosylation profiles of these glycoproteins can be used as prognostic tools for colorectal cancer.