MSACL 2018 EU Abstract

Self-Classified Topic Area(s): Practical Training

Calibrators and Internal Standards in Protein Mass Spectrometry Assays: Differences, Commonalities, and Best-Practice

Christopher M. Shuford (1)
(1) Laboratory Corporation of America, Burlington, NC, USA

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Christopher Shuford (Presenter)
Laboratory Corporation of America

Relevant Financial Disclosures (within past 24 months, reported on Sep 14, 2021)

Stock/Bonds Laboratory Corporation of America

Salary Laboratory Corporation of America

Discussing Products? NO, presenter will NOT present educational content related to products/services of the ineligible company(ies) listed above.

Conflict mitigation NOT REQUIRED.

Abstract

Internal standards are not calibrators. This fact is often overlooked in the field of proteomics, which has propagated misconceptions as new protein mass spectrometry assays are translating into the clinical chemistry lab. In the first part of this 3 part series, the distinction between calibrators and internal standard will be highlighted in the context of protein measurements by mass spectrometry. In the second part of the series, best practices for calibration and standardization/harmonization of protein mass spectrometry assays will be discussed. Finally, options for internal standardization of protein mass spectrometry assays will be reviewed and contrasted in the third part of the series.