MSACL 2018 EU Abstract

Self-Classified Topic Area(s): Endocrinology

Quantification of Testosterone, Androstenedione and 17-Hydroxyprogesterone Collected Using Mitra Micro Sampling Devices

David Marshall, Brian Keevil
Manchester University NHS Foundation Trust

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David Marshall (Presenter)
Manchester University NHS Foundation Trust

Presenter Bio: I am a trainee Clinical Scientist at Wythenshawe Hospital in Manchester. I have worked with clinical mass spectrometry for the last 3 years, including routine operation, method development and troubleshooting. I am also in the process of completing a Masters in Clinical Science as part of my training

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Grant/Research Support Mr

Salary Waters

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Abstract

Mitra devices are small polymer tips that absorb a fixed amount of blood which can be dried prior to analysis.

A simple liquid:liquid extraction was used following reconstitution of the mitra device in distilled water. Separation was carried out using a Waters Acquity HSS T3 column which resolved the peaks well.

We have developed an assay for the simultaneous quantification of testosterone, androstenedione and 17-hydroxyprogesterone using a single mitra tip.