MSACL 2018 EU Abstract

Global Glycoproteomic Analysis of O-glycosylation in Human Herpesviruses

Ieva Bagdonaite (1) , Rickard Nordén (2), Hiren J. Joshi (1), Sarah L. King (1), Sergey Y. Vakhrushev (1), Sigvard Olofsson (2), and Hans H. Wandall (1)
(1) Copenhagen Center for Glycomics, University of Copenhagen, Copenhagen, Denmark (2) Department of Infectious Diseases, University of Gothenburg, Gothenburg, Sweden

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Ieva Bagdonaite (Presenter) University of Copenhagen

Relevant Financial Disclosures (within past 24 months, reported on Aug 01, 2021) No relevant financial relationship(s) to disclose. Discussing Products? NO, presenter will NOT present educational content related to products/services of the ineligible company(ies) listed above. Conflict mitigation NOT REQUIRED.

Information on site-specific O-glycosylation of viral envelope glycoproteins is generally very limited despite important functions. We present a powerful mass-spectrometry based strategy to globally identify O-glycosylation sites on viral envelope proteins of a given virus in the context of a productive infection. We successfully utilized the strategy to map O-linked glycosylation sites on several complex herpesviruses demonstrating that O-glycosylation is widely distributed on most envelope proteins. Moreover, we used genetically engineered keratinocytes lacking O-glycan elongation capacity to demonstrate that O-linked glycans are indeed important for HSV-1 biology as HSV-1 particles produced in these cells had significantly lower titers compared to wild-type keratinocytes. These tools enable wider discovery and detailed analysis of the role of site-specific O-glycosylation in virology.