MSACL 2019 EU Abstract

B Vitamin Reference Ranges Determination Using HPLC-MS/MS and Retrospective Statistical Analysis

Sadykov Arseny, Ilgar Mamedov, Zolkina Irina, Aushkap Sergey, Darya Sevko, Yurasov Vasiliy
Scientific laboratory "Chromolab", Moscow, Russia

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Arseny Sadykov (Presenter) Scientific laboratory "Chromolab", Moscow, Russia >> POSTER (PDF)

Presenter Bio: MD in medical genetics. Researcher in scientifit laboratory "Chromolab".

Relevant Financial Disclosures (within past 24 months) No relevant financial relationship(s) to disclose. Conflict mitigation NOT REQUIRED.

Introduction

Determination of the correct reference intervals (RIs) is the basis for precise interpretation of the results in routine laboratory testing. The use of RIs established in other laboratories is often incorrect due to different populations and methods. Ideally, each laboratory should have its own RIs, however their determination is very costly for many laboratories. One of the compromise solutions is a retrospective mathematical analysis of routine tests for determining RIs. At present, there is no rigorous mathematical algorithm that would allow the RI to be set retrospectively from the results of routine research: in most methods, the researcher often has to use visual assessment or he faces not always the obvious choice.

Objectives

The objectives were to establish reference ranges for group B vitamins in the Caucasian population in whole blood and plasma using various methods proposed in statistics, evaluation of their advantages and disadvantages and develop clear criteria for choosing a particular method.

Methods

In this study, we have used HPLC/MS/MS method for determining the concentration of vitamins in whole blood and plasma. For retrospective data analysis and establishing reference ranges we have used and compared Bhattacharya, Hoffmann and Mixed Gaussian Models methods.

Results

664 patients were included. We established RIs for Caucasian population. For plasma: vitamin B2 (FAD) 56-97 nmol/l, vitamin B2 (riboflavin) 4-43 nmol/l, vitamin B3 (niacin) 13-161 nmol/l, vitamin B5 (pantothenic acid) 54,5 – 604,4 nmol/l, vitamin B6 (PLP) 11,3- 302 nmol/l, vitamin B7 (biotin) 0,025-5,647 nmol/l. For whole blood: vitamin B1 (TPP) 82 – 239 nmol/l, vitamin B2 (FAD) 116 – 393 nmol/l, vitamin B6 (PLP) 3,5 – 80 nmol/l.

Conclusion

At the moment there is no universal method for determining reference intervals. Depending on the data structure, each method has its own advantages and disadvantages. The most optimal in most cases was the Hoffman method presumably due to the best excluding from a sample people who take B vitamins as dietary supplements.