L. Renee Ruhaak Department of Clinical Chemistry and Laboratory Medicine, Leiden University Medical Center, Leiden, The Netherlands.
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L.Renee Ruhaak (Presenter) Leiden University Medical Center
Relevant Financial Disclosures
(within past 24 months)
No relevant financial relationship(s) to disclose.
Abstract
Key take-aways:
- Knowledge of different types of calibration strategies
- Basic understanding of metrological traceability chain
- Importance of a defined measurand and implications of proteoforms
Description
Wouldn’t it be nice if a test result would be the same, independent of the laboratory or method that was used to obtain the test result? Accurate quantitation of proteins starts with proper calibration, and proper calibration starts with the correct internal standard. This session briefly discussed the selection of internal standards as well as some specifics of their use and role in calibration. Then, the development of a proper calibration strategy, specifically the choice of calibrators as well as how they can and should be anchored to achieve metrological traceability is outlined. The need for a well-defined measurand, specifically in light of the heterogeneity observed by multiple proteoforms, and the implications of heterogeneity for the development of calibration strategies will be explained.
= Discovery stage.
= Translation stage.
= Clinically available.
