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Abstract INTRODUCTION: Over the last decade, liquid chromatography coupled to mass spectrometry (LC-MS) has gained widespread popularity and transitioned from research to routine clinical laboratories. While most clinical research laboratories use triple quadrupole mass spectrometers combined with liquid chromatography (LC-MS/MS), for targeted, selective and sensitive quantitation, an increasing number of laboratories prefer one single MS that can efficiently perform routine quantitation of analytes and also screening, confirm, and characterize unknown analytes (ranging from small to large sizes). As a result we observe increasing demands for ready-to-use complete kits for small molecules running on high resolution instruments.
OBJECTIVES: In this study, we report benefits and limitations faced during implementation of RECIPE ClinMass® LC-MS/MS complete kits on a Thermo ScientificTM Q Exactive PlusTM coupled to a Thermo ScientificTM VanquishTM Duo system for Research Use Only.
METHODS: Different detection modes (FullMS, tSIM, PRM) were tested. A complete verification of all kits was carried out, performance was compared to results from a Thermo ScientificTM EnduraTM coupled to a Thermo ScientificTM TranscendTM II system. We provide results from RECIPE’s MS7000 25-OH-Vitamin D2/D3 kit and MS9000 TDM Platform.
RESULTS AND CONCLUSION: In conclusion, our Q Exactive verification data demonstrated good analytical performance meeting research laboratory requirements. |
= Discovery stage.
= Translation stage.
= Clinically available.
