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Abstract Building multi-analyte LC-MS assays is a common temptation in Laboratories who are often faced with the drive to do “more with less”. This is especially true when the analytes in question are related clinically and share similar phys/chem properties. Adding these extra analytes can seem like additional data for free. However, in addition to extra logistical requirements (Increased cals, QCs, QA samples, data review, troubleshooting) there are analytical challenges to overcome such as differing stabilities and recoveries, different relative sensitivities, and the purity of reference standards that raise the question – how free is it really?
This session will comprise of an introduction into multi-analyte methods and their limitations, followed by a case study of the development of a LC-MS assay for the measurement of five atypical anti-psychotics, with a final practical session where the audience will be split into groups to discuss theoretical examples.
Take home ‘pearls’
• Being able to identify the common pitfalls with multi-analyte methods and how to avoid falling into them
• Knowing the steps that can be taken to assess potential issues during method development
• An understanding of the limitations of large multi-analyte methods and the fundamental issues with demonstrating control over assays of this nature.
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= Discovery stage.
= Translation stage.
= Clinically available.
