MSACL 2019 EU Abstract

Steroidomics Profile Analysis by LCHR-MS in Human Seminal Fluid

Eulàlia Olesti(1,2), Arnaud Garcia(1,2), Víctor González-Ruiz(1,2), Rita Rahban(2,3), Julien Boccard(1,2), Serge Nef(2,3), Serge Rudaz(1,2)
(1) Analytical Sciences, School of Pharmaceutical Sciences, University of Geneva, University of Lausanne, Rue Michel-Servet 1, 1206 Geneva, Switzerland. (2) Swiss Centre for Applied Human Toxicology (SCAHT), Switzerland. (3) Department of Genetic Medicine and Development, Faculty of Medicine, University of Geneva, Rue Michel-Servet 1, 1206 Geneva, Switzerland.

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Eulalia Olesti (Presenter) University of Geneve

Presenter Bio: Dra. Eulalia Olesti studied pharmacy in the University of Barcelona (2009-2014), where she also got a master in Neurosciences (2015) while gaining experience in several healthcare centers and research laboratories. She holds a PhD in pharmacology and bioanalysis (2018) from the Universitat Pompeu Fabra, obtained under the supervision of Dr. Rafael de la Torre and Dr. Óscar Pozo and funded by the European Comission. During this period, she acquired a strong background in toxicological bioanalysis, clinical pharmacology and metabolomics while working on translational metabolomics studies and phase I clinical trials. She extensively developed bioanalytical methodologies to quantify endogenous and exogenous compounds in biological matrices with chromatographic techniques coupled to mass spectrometry.
In 2019 she joined the School of Pharmaceutical Sciences of the University of Geneva as a post-doctoral researcher under the direction of Prof. Serge Rudaz. She is actively collaborating with the Swiss Centre for Applied Human Toxicology (SCAHT) and during her career, she has published 10 scientific articles in peer-review journals (6 of them as first author). Her current research interests are analytical toxicology, clinical pharmacology and metabolomics bioanalysis.

Relevant Financial Disclosures (within past 24 months) No relevant financial relationship(s) to disclose. Discussing Products? NO, presenter will NOT present educational content related to products/services of the ineligible company(ies) listed above. Conflict mitigation NOT REQUIRED.

INTRODUCTION: Steroids play a crucial role in homeostasis of many biological processes including spermatogenesis, thus being responsible for some male infertility issues. Although steroids have been largely studied in many biological matrices (such as urine and plasma), there is very limited information of the steroid content in seminal liquid and its potential study as potential indicators of male infertility and other conditions.
OBJECTIVES: In this study, a LC-HRMS strategy has been developed in order to obtain the steroidomic profile of human seminal fluid.
METHODS: A comparison between supported liquid extraction (SLE) and solid liquid extraction (SPE) was carried out, and the chosen SPE method further optimized to map the largest possible number of compounds. Steroids were identified by using DynaStI, a publicly available retention time prediction webtool developed in our lab, to match the experimental data (i.e. accurate mass and tR).
RESULTS: Altogether these resources allowed us to develop a post-targeted approach able to consistently detect 40 steroids in seminal plasma (with half of them being androgens). Such steroidal profile was stable across different extraction times and injection days. In addition to accurate mass and retention time, the identity of 70% of the steroids detected in such steroidomic profile was confirmed by comparing their fragmentation patterns in real samples to those of standards. Finally, the workflow was applied to compare and distinguish the steroidomics profile in seminal liquid from healthy volunteers (n = 6).
CONCLUSION: In all, the developed steroidomics strategy allows to reliably monitor an extended panel of 40 steroids in human seminal fluid.