MSACL 2019 US Abstract

Self-Classified Topic Area(s): Tissue Imaging

Rapid Evaporative Ionization Mass Spectrometry Explores Tumor Necrosis and Tissue Autolysis

Pierre-Maxence Vaysse1,2,3, Heike I. Grabsch4,5, Mari F.C.M. van den Hout4, Steven W.M. Olde Damink2, Ron M.A. Heeren1, Tiffany Porta Siegel1
(1) Maastricht Multimodal Molecular Imaging Institute (M4I), Division of Imaging Mass Spectrometry, Maastricht University, The Netherlands (2) Department of Surgery, Maastricht University Medical Center+, The Netherlands (3) Department of Otorhinolaryngology, Head & Neck Surgery, Maastricht University Medical Center+, The Netherlands (4) Department of Pathology, GROW School for Oncology and Developmental Biology, Maastricht University Medical Center+, The Netherlands (5) Division of Pathology and Data Analytics, Leeds Institute of Medical Research at St James’s, University of Leeds, United Kingdom

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Pierre-Maxence Vaysse (Presenter)
Maastricht University

Presenter Bio: PhD student, M4I

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No relevant financial relationship(s) to disclose.

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Abstract

Lipid profiling has gained major interest with the emergence of mass spectrometry technologies for tissue classification in clinical research. While characterizing cell death can be relevant in surgical pathology, necrosis markers, such as ceramide d34:1, have already been highlighted in previous intra-tumor heterogeneity studies. We use REIMS to distinguish vital tumor and necrosis in human tumors and to detect autolysis changes using porcine tissue as a model. Here, we highlight two mass peaks at m/z 572.4 (ceramide d34:1) and 682.5 as major contributors to separate viable tumor and necrosis in liver metastases (11 patients, 127 profiles, correct classification rate 97.8%). Both mass peaks were also characteristic of autolysis (120 profiles, correct classification rate 95%) illustrating their potential broader role as biomarkers of cell death.