MSACL 2019 US Abstract

Self-Classified Topic Area(s): Tox / TDM / Endocrine

Analysis of Steroid Hormones in Serum and Follicular Fluid of Female Rhesus Macaques with Chronic Combined Hyperandrogenemia and/or Western-Style Diet

David W. Erikson (1), Cecily V. Bishop (1), Steven W. Blue (1), Amy V. Kaucher (1), Ov D. Slayden (1), Jon D. Hennebold (1), Richard L. Stouffer (1)
(1) Oregon National Primate Research Center, Beaverton, OR

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David Erikson (Presenter)
Oregon National Primate Research Center

Presenter Bio: David Erikson, Ph.D. is the Director of the Endocrine Technologies Core at Oregon National Primate Research Center. Dr. Erikson received his Ph.D. in Physiology from Penn State University in 2006. He then completed postdoctoral training at Texas A&M University (2006-2010) and UCSF (2010-2012) before taking a position as a Research Specialist at UCSF (2012-2014). Dr. Erikson has been in his current position since 2014.

Relevant Financial Disclosures (within past 24 months)

Grant/Research Support National Institutes of Health

Discussing Products? NO, presenter will NOT present educational content related to products/services of the ineligible company(ies) listed above.

Conflict mitigation NOT REQUIRED.

Abstract

Liquid chromatography-tandem mass spectrometry (LC-MS/MS) has become the method of choice for analyzing steroid hormones in many laboratories. We have developed a method for the simultaneous measurement of seven steroid hormones (androstenedione, cortisol, cortisone, estradiol, estrone, progesterone, and testosterone) using a small sample volume and simple sample preparation protocol. The method is also sensitive and offers a wide analytical range making it ideal for routine analysis of research samples. Application of the method to serum and follicular fluid samples from female rhesus macaques with chronic combined hyperandrogenemia and/or western-style diet revealed differences in the steroid profile when compared to the steroid profile of women with polycystic ovary syndrome (PCOS).