MSACL 2019 US Abstract

Strategies for Developing High-Throughput Mass Spectrometry Analyses of 25-Hydroxyvitamin D

Nicholas R. Oranzi1, Jiajun Lei1, Calvin Wiese2, and Richard A. Yost1,3
1 Department of Chemistry, University of Florida, 2 Wellspring Clinical, 3 Department of Pathology, Immunology, and Laboratory Medicine, University of Florida

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Richard Yost (Presenter) University of Florida

Presenter Bio: Rick Yost is University Professor and Head of Analytical Chemistry at the University of Florida. He received his BS in Chemistry in 1974 from the University of Arizona and his PhD in Analytical Chemistry in 1979 from Michigan State University. He has served on the faculty of the University of Florida for almost 40 years.

Rick’s professional activities have focused on research and teaching in analytical mass spectrometry, particularly tandem mass spectrometry (MS/MS). He and his group build unique instruments and apply them to areas such as clinical and environmental analysis, metabolomics, and forensic chemistry. Rick has supervised the research of over 100 PhD students at UF. He recently completed terms on the Florida Board of Governors (Regents) and the UF Board of Trustees. He is also a Professor of Pathology at both the University of Florida and the University of Utah. His research has been recognized with the 1993 ASMS Award for Distinguished Contribution in Mass Spectrometry and the 2018 MSACL Award for Distinguished Contribution to Clinical Mass Spectrometry.

Relevant Financial Disclosures (within past 24 months) Grant/Research Support Wellspring Clinical Discussing Products? YES, presenter will present educational content related to products/services of one or more ineligible company(ies) listed above. Conflict mitigation IN PROCESS.

To meet the rising demand for vitamin D testing, new strategies are required for increasing the throughput of 25-hydroxyvitamin D analysis by mass spectrometry. Ion mobility mass spectrometry is shown to separate 25-hydroxyvitamin D from its interfering stereoisomer in milliseconds, instead of minutes by traditional liquid chromatography. Coupling ion mobility with LC/MS leads to substantial decrease in sample analysis time. Additionally, sample introduction strategies were explored for coupling mass spectrometry with offline sample separation and storage. Segmented flow offers potential advantages for improving throughput and optimizing sample preparation and electrospray ionization.