MSACL 2019 US Abstract

Developing Laser Assisted Rapid Evaporative Ionization Mass Spectrometry (LA-REIMS) as an Automated High-Throughput Platform for Clinical Microbiology

Simon Cameron (1), Alvaro Perdones-Montero (1), Adam Burke (1), Kate Alexander-Hardiman (1), Alireza Abdolrasouli (1,2), Monica Rebec (2); Zoltan Takats (1).
(1) Imperial College London, London, UK (2) Imperial College Healthcare NHS Trust, London, UK

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Alvaro Perdones-Montero (Presenter) Imperial College London

Relevant Financial Disclosures (within past 24 months) Grant/Research Support Waters Corporation Discussing Products? YES, presenter will present educational content related to products/services of one or more ineligible company(ies) listed above. Conflict mitigation IN PROCESS.

Unlike commercially available MS platforms, rapid evaporative ionization mass spectrometry (REIMS) does not require sample preparation, such as the addition of a matrix, nor extraction prior to analysis. Laser assisted REIMS utilises a carbon dioxide laser for biomass heating and evaporation; producing gas-phase ions which are aspirated directly to a mass spectrometer for real-time analysis. LA-REIMS is an ideal technique for both automated and high-throughput applications – both of which are increasingly essential for clinical microbiology laboratories. This presentation will detail the on-going work to develop REIMS for clinical microbiology including (1) development of direct-from-culture identification for bacteria, yeast, and filamentous fungi, (2) rapid antimicrobial susceptibility testing, and (3) pathogen detection directly from clinical samples.