MSACL 2019 US Abstract

Multimodal Imaging Mass Spectrometry Technologies to Visualize Staphylococcal Molecular Adaptation within the Infectious Microenvironment

William J. Perry (1,2,3), Caroline M. Grunenwald (3,4), Jessica R. Sheldon (3,4), Eric P. Skaar (3,4), Jeffrey M. Spraggins (1,2,5), and Richard M. Caprioli (1,2,5,6,7)
(1) Mass Spectrometry Research Center, Vanderbilt University, Nashville, TN, United States (2) Department of Chemistry, Vanderbilt University, Nashville, TN, United States (3) Vanderbilt Institute for Infection, Immunology, and Inflammation, Vanderbilt University, Nashville, TN, United States (4) Department of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, Nashville, TN, United States (5) Department of Biochemistry, Vanderbilt University, Nashville, TN, United States (6) Department of Pharmacology, Vanderbilt University, Nashville, TN, United States (7) Department of Medicine, Vanderbilt University, Nashville, TN, United States

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William Perry (Presenter) Vanderbilt University

Presenter Bio: William J. Perry is currently a graduate student in Dr. Richard Caprioli's laboratory at Vanderbilt University. His dissertation work focuses on developing strategies to identify staphylococcal adaptive molecular responses from within the infection environment.

Relevant Financial Disclosures (within past 24 months) Grant/Research Support Vanderbilt University Discussing Products? NO, presenter will NOT present educational content related to products/services of the ineligible company(ies) listed above. Conflict mitigation NOT REQUIRED.

Imaging Mass Spectrometry (IMS) is a technology that allows for molecular species to be measured directly from tissue while preserving spatial distributions. Utilizing fluorescence microscopy and Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry (MS), we have acquired images of S. aureus molecular distributions within the infection environment. Instrumental modifications and novel tissue preparation strategies have allowed for the visualization of multiple classes of bacterial specific bio-molecules attributed to adaptive survival of S. aureus within the host. Using multimodal imaging technologies, we have verified the presence and distributions of these bacterial biomolecules, never before observed in vivo, co-localizing with the infectious abscess microenvironment, providing new targets to study and treat bacterial pathogenesis.