MSACL 2019 US Abstract

A LC-MS/MS Method using Antibody Free Sample Preparation for Multiplexed Targeted Quantitative Intact Protein Biomarkers of Diabetes from Human Serum

Jesse C. Seegmiller, Brian J. Wolfe, David J. Schmit
Advanced Research and Diagnostic Laboratory, Department of Laboratory Medicine and Pathology, University of Minnesota, 1200 Washington Avenue S., Minneapolis, MN 55415

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Jesse Seegmiller (Presenter) University of Minnesota

Presenter Bio: Dr. Seegmiller is a board certified clinical chemist at the University of Minnesota in the Department of Laboratory Medicine and Pathology. He focuses his research activities on esoteric testing where he primarily employs mass spectrometry to analyze and develop methods for biological samples at the University's Advanced Research and Diagnostics Laboratory (ARDL).

Relevant Financial Disclosures (within past 24 months) Grant/Research Support NIH Discussing Products? NO, presenter will NOT present educational content related to products/services of the ineligible company(ies) listed above. Conflict mitigation NOT REQUIRED.

The clinical utility and measurement of insulin in most immunometric assays is complicated by the known cross-reactivity with insulin analog therapies used by many diabetic patients. Thus, a more specific and comprehensive protein biomarker panel for diabetes is highly desirable for clinicians to both diagnose, and study disease progression. Here, we discuss the development of an antibody free solid phase sample preparation method to measure intact insulin, C-peptide, glucagon, and somatostatin 14 by liquid chromatography tandem mass spectrometry. All of which play roles in the absorption and formation of glucose in vivo.