MSACL 2019 US Abstract

Rapid Bile Acid Isomer Separation with Ion Mobility-Mass Spectrometry

Robin H.J. Kemperman, Richard A. Yost
Department of Chemistry, University of Florida, Gainesville, FL, USA

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Robin Kemperman (Presenter) University of Florida

Presenter Bio: Robin Kemperman received his BASc in chemistry from the HAN University of Applied Sciences in The Netherlands. Thereafter, he fulfilled his MSc in analytical chemistry at the University of Florida. Currently, Mr. Kemperman is a Ph.D. Candidate in analytical chemistry at the University of Florida and works under the direction of Dr. Richard Yost. His work has covered a variety of aspects in mass spectrometry (MS) over the past 4 years, including targeted analysis using triple quadrupole MS, high-field asymmetric-waveform ion mobility spectrometry (FAIMS) and classic drift tube ion mobility spectrometry (DTIMS), both in combination with MS. He currently works on new approaches for metabolite profiling using DTIMS paired with LC-MS. His research interests are focused on novel innovations for biomedical and clinical applications.

Relevant Financial Disclosures (within past 24 months) Grant/Research Support Agilent Discussing Products? NO, presenter will NOT present educational content related to products/services of the ineligible company(ies) listed above. Conflict mitigation NOT REQUIRED.

Ion mobility spectrometry (IMS) is a powerful tool because of its rapid separation of isobars and isomers that are unresolved by traditional LC-MS and can cause complications in clinical methods. IMS is an emerging technology in the fields of metabolomics and clinical analysis, for both targeted assays and global studies. This study will demonstrate how IMS can assist in rapid separation of isomeric bile acids and examine its potential use in the clinical field. Several separation strategies were applied using two different IMS platforms, a drift tube IMS and a high-field asymmetric-waveform IMS, both coupled to a mass spectrometer.