MSACL 2019 US Abstract

Quantification of Superwarfarin Rodenticides in Plasma using High-Performance LC-MS/MS

Daniel G. Nosal (1), Douglas L. Feinstein (2), and Richard B. van Breemen (1)
(1) Linus Pauling Institute, Oregon State University (2) Department of Anesthesiology, University of Illinois at Chicago

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Daniel Nosal (Presenter) Oregon State University - Linus Pauling Institute

Presenter Bio: Daniel Nosal is a fifth-year PhD student at Oregon State University (OSU) Department of Pharmaceutical Sciences – Linus Pauling. He received a bachelor’s degree in chemistry from the University of Illinois at Chicago. At OSU, in the lab of Dr. Richard van Breemen, Daniel developed proficient skills in mass spectrometry based quantitative analysis and natural product drug discovery by leading a diverse set of projects. Examples include, quantifying bioactive components of botanicals and characterizing metabolites of potential pharmaceuticals. Daniel has also gained experience working on pulsed ultra-filtration and affinity selection mass spectrometry-based drug discovery assays for the estrogen and progesterone receptors. He is now focused on understanding the pharmacokinetics of long-acting anticoagulant rodenticides, superwarfarins, in a clinical setting, using mass spectrometry.

Relevant Financial Disclosures (within past 24 months) No relevant financial relationship(s) to disclose. Discussing Products? NO, presenter will NOT present educational content related to products/services of the ineligible company(ies) listed above. Conflict mitigation NOT REQUIRED.

Superwarfarins are long-acting anticoagulant rodenticides (LAARs) that are orders of magnitude more potent than warfarin and function by inhibiting the vitamin K cycle thereby preventing the function of vital clotting factors and other vitamin K dependent proteins. Prolonged human exposure to LAARs can lead to various coagulopathy disorders, neurological damage and death. For poisoning victims, it is vital to know the extent of LAAR exposure to guide coagulopathy therapy. We have developed and validated a rapid, sensitive, and selective LC-MS/MS method to separate and quantitate diastereoisomeric pairs of the common LAARs brodifacoum, difenacoum, and bromadiolone in human plasma.