Intraoperative-MS-based Biopsy Analysis Platform for Glioma Diagnosis, Extent of Tumor Cell Infiltration Estimation, and IDH Subtyping
Clint Alfaro(1); Valentina Pirro(1); Alan Jarmusch(1); Eyas Hattab(2); Aaron Cohen-Gadol(3); Robert Graham Cooks(1) (1) Purdue University, (2) University of Louisville School of Medicine, (3) Goodman Campbell Brain and Spine, Indiana University School of Medicine
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Clint Alfaro (Presenter) Purdue University
Presenter Bio: I am currently a fifth year PhD student in Prof. Graham Cooks' lab at Purdue University. My PhD work has focused on the molecular diagnosis of cancer tissue using ambient ionization mass spectrometry. I was awarded a TL1 predoctoral training award through the Indiana Clinical and Translational Sciences Institute to support our project focusing on performing DESI-MS in the operating room to diagnose brain cancer (glioma), chemically characterize resection margins, and assess mutations in isocitrate dehydrogenase, an important prognostic marker for gliomas. I attended the University of North Carolina at Greensboro from 2010-2014, and graduated with a B.S. degree in Biology and Biochemistry. While there, I worked in the lab of Prof. Nadja Cech as an undergraduate researcher for 2 and a half years studying electrospray ionization fundamentals.
Relevant Financial Disclosures
(within past 24 months)
No relevant financial relationship(s) to disclose.
Abstract
Gliomas are high morbidity brain tumors that microscopically infiltrate brain tissue, preventing a surgical cure; however, studies show that maximizing gross tumor resection improves patient survival. We hypothesized that a significant clinical need could be satisfied by an objective system for rapid surgical margin assessment. Herein, the intraoperative molecular profiling of stereotactic brain tissue biopsies with desorption electrospray ionization mass spectrometry (DESI-MS) was used to evaluate the degree of tumor infiltration from fresh tissue smears. N-acetylaspartic acid, phospholipid profiles, and 2-hydroxyglutarate were measured to assess tumor cell percentage, disease status, and isocitrate dehydrogenase mutation status, respectively. Our preliminary study demonstrates DESI-MS as a method for rapid and objective assessment of glioma during tumor resection.
= Discovery stage. (17.55%, 2019 US)
= Translation stage. (42.72%, 2019 US)
= Clinically available. (39.74%, 2019 US)
