MSACL 2019 US Abstract

Mass Spectrometry-based Quantitative Diagnostic Test of Allograft Kidney using Targeted Proteome Profiling of FFPE Specimens

Lei Song (1), Fei Fang (1), Gang Zeng(7), Peng Liu (4), Hongda Liu (1), Yang Zhao (1), George C. Tseng (4,5,6), Parmjeet Randhawa (7), Kunhong Xiao (1,2,3)
(1) Department of Pharmacology and Chemical Biology; (2) Vascular Medicine Institute; (3) Biomedical Mass Spectrometry Center, School of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania 15261, USA; (4) Departments of Biostatistics, (5) Human Genetics and (6) Computational Biology, University of Pittsburgh; (7) Department of Pathology, Division of Transplantation Pathology, The Thomas E Starzl Transplantation Institute, University of Pittsburgh

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Kunhong Xiao (Presenter) University of Pittsburgh

Presenter Bio: I am Associate Professor in the Department of Pharmacology and Chemical Biology; Vascular Medicine Institute; and Biomedical Mass Spectrometry Center, School of Medicine, University of Pittsburgh.

Relevant Financial Disclosures (within past 24 months) No relevant financial relationship(s) to disclose. Discussing Products? NO, presenter will NOT present educational content related to products/services of the ineligible company(ies) listed above. Conflict mitigation NOT REQUIRED.

The transcriptomic and proteomic analyses through formalin-fixation and paraffin-embedding (FFPE) specimens reflect systemic and inherent renal injury perturbations for different kidney transplant injury states. In this study, we developed a high-sensitivity and high-confidence workflow to map transcriptome and proteome changes from the limited amount of FFPE sample after kidney transplantation. Based on the transcriptomic and proteomic biomarkers obtained from patients with different injury states, we developed a robust multiple reaction monitoring (MRM)-based assay which can be implemented in clinic for transplant monitoring. The application of this quantitative diagnostic test can mitigate the shortcomings of conventional examination using eyeballed histologic opinions.