MSACL 2019 US Abstract

Multi-Point Internal Calibration: IntrinsiX LC-MS/MS Analysis of Methotrexate

Melissa Hoffman (1), Michael Schmeling (2), Jayme Dahlin (3), Nicholas Bevins (1), Petr Jarolim (3), Robert Fitzgerald (1), Andrew Hoofnagle (2)
(1) University of California, San Diego, CA (2) University of Washington, Seattle, WA (3) Brigham and Women

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Melissa Hoffman (Presenter) University of California, San Diego

Presenter Bio: Melissa Hoffman, Ph.D. is a Clinical Chemistry Fellow at UC San Diego Center for Advanced Laboratory Medicine, under the direction of Dr. Robert Fitzgerald. She attended the University of South Florida where she received her Ph.D. in Cancer Biology. Her graduate work was performed at the Moffitt Cancer Center under Dr. John Koomen’s mentorship, where she was trained in mass spectrometry. She applied her interdisciplinary background to her dissertation research, which focused on using quantitative proteomics to support translational cancer research with an emphasis on personalized cancer diagnostics development. In her current fellowship, she is expanding her skills in the areas of small molecule analysis and clinical method validation and in the future hopes to apply these skills to IVD development for improved disease monitoring.

Relevant Financial Disclosures (within past 24 months) No relevant financial relationship(s) to disclose. Discussing Products? NO, presenter will NOT present educational content related to products/services of the ineligible company(ies) listed above. Conflict mitigation NOT REQUIRED.

Clinical LC-MS/MS assays require batching of samples and often includes calibration curve generation with each batch. Conventional calibration strategies increase costs and turnaround times, presenting barriers for clinical LC-MS/MS. To address the need for alternative approaches, we present a novel multi-point internal calibration strategy, termed IntrinsiX (Waters), that eliminates the need for batch-mode analysis and costly external calibrator runs. The IntrinsiX LC-MS/MS method for methotrexate analysis in patient serum was validated in a multi-site, proof-of-concept study demonstrating assay robustness and suggesting easy implementation for therapeutic drug monitoring in a standard clinical lab.