= Discovery stage. (53.14%, 2025)
= Translation stage. (22.33%, 2025)
= Clinically available. (24.53%, 2025)

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MSACL 2025 : van der Gugten

MSACL 2025 Abstract

Self-Classified Topic Area(s): Practical Training > none > none

Challenges of Dried Blood Spot Applications

Grace van der Gugten (1), Maryam Salehi (2)
(1) Provincial Health Services Authority, Vancouver, Canada, (2) Emory University, Atlanta, GA

Grace van der Gugten, B.Sc. Chemistry (Presenter)
Provincial Health Services Authority, BCCDC Toxicology Lab

Presenter Bio: Grace discovered her love for clinical mass spectrometry when she began working at St Paul's Hospital in Vancouver in the special chemistry mass spec group with Dr. Dan Holmes in late 2010. Grace was challenged in this role but gained a wealth of knowledge and experience over her 10+ years in the SPH laboratory. She puts this experience and knowledge into use in her current role as Mass Spectrometry Lab Scientist in the Toxicology Lab at the BCCDC in Vancouver, BC. Grace loves developing streamlined, easy to use (if possible!) clinical mass spectrometry assays; teaching others and helping others succeed; and troubleshooting (especially when the problem is solved!).

Relevant Financial Disclosures (within past 24 months, reported on Mar 05, 2026)
No relevant financial relationship(s) to disclose.


Maryam Salehi, PhD, MS, NRCC, FADLM (Presenter)
Emory University School of Medicine

Presenter Bio: Maryam Salehi is a board-certified clinical chemist. She earned her PhD in Biomedical Sciences from the University of South Dakota in 2017. Later that year Dr. Salehi joined the Emory University Department of Pathology where she completed a postdoctoral fellowship in Clinical Chemistry in 2019. Following that, Maryam joined CDC’s Newborn Screening Laboratories and worked as a clinical chemist focusing on method development and validation of mass spectrometry based NBS testing, as well as production and evaluation of NBS quality control and proficiency testing materials. Dr. Salehi then joined Phoenix Children’s as an Assistant Professor and the Director of Special Chemistry where she brought up new clinical services including hemoglobinopathy service and autoimmunity testing. She also drove the setup of a new mass spectrometry laboratory from the ground up. In 2024, Maryam joined the Department of Pathology at Emory University School of Medicine as an Assistant Professor of Pathology; she also serves as the Medical Director of Toxicology and Associate Medical Director of Clinical Chemistry at Grady Health System which is a level 1 trauma center in Atlanta. She currently focuses on setting up a mass spectrometry laboratory section to bring toxicology confirmatory testing to Grady. Maryam is passionate about teaching pathology residents, medical students and laboratory staff, advancing her career in clinical toxicology area, and building new high complexity clinical lab services from the ground up.

Relevant Financial Disclosures (within past 24 months)
Not yet reported.

Abstract

There are benefits to dried blood spots (DBS) as a sample type, but quantitative LC-MS/MS analysis using DBS has its own particular challenges and limitations. DBS are primarily used in newborn screening (NBS) programs, and this session will focus on applications in NBS with a brief discussion of potential applications to other clinical lab disciplines. Challenges that will be discussed in this session will include:
-Preparation of calibrator, quality control (QC) and proficiency testing (PT) samples.
-Characterization of the QC and PT material.
-Preparing blank matrix.
-Hematocrit effects.
-Blood distribution on filter paper.
-Extraction techniques.
-Internal Standard addition/limitations.
-Storage and transport of DBS.
-Reference material certification.
Case studies including CDC QC/PT materials preparation and failures and successes in an LDT Congenital Adrenal Hyperplasia (CAH) steroid panel as well as an amino acids-acylcarnitines panel will be discussed. Current developments in microsampling will be briefly covered.

Take Home Pearls:
1. Learn about the steps and considerations for reference material preparation in house.
2. Why DBS calibrator and QC preparation is so challenging, and how to be successful when preparing in house materials.
3. Describe the applications of NBS in clinical practice.