Podium Presentation in Room 4 on Thursday at 13:35 (Chair: Christopher Anderton / Wojciech Michno)
Authors: David C. Muddiman and Erin S. Baker
The mass spectrometry imaging field is moving into the realm of clinical analysis and this is largely based on MALDI and DESI ionization methods. We have developed a novel ionization method called Infrared Matrix Assisted Laser Desorption Electrospray Ionization (IR-MALDESI) which can be readily adaptable to many different sample types.
Demonstrate the effectiveness of IR-MALDESI for a wide-variety of pre-clinical applications and new directions using different mass spectrometry platform technologies.
A home-built IR-MALDESI sources has been coupled to high resolution accurate mass (Orbitrap) and drift tube ion mobility time-of-flight mass spectrometers. A diverse array of sample types will be presented from HTS/HCS, bones and diverse array of tissues.
For HTS/HCS screening applications, zero sample preparation is required from very complex mixtures with the throughput of 2 samples per second. For bones, we developed a method that does not require decalcification prior to imaging which is critical because HCl will degrade/alter molecular information present in the sample. Finally, a wide range of ongoing studies will be presented including anti-malaria medications, HIV drugs and their co-localization with viral RNA and immune cells, how a fetus responds to exposure to fire retardants.
IR-MALDESI has demonstrated to be extraordinarily adaptable to different sample types and requires no sample preparation. This has allowed us to carry out mass spectrometry imaging for a wide-range of applications that relate to human health and exposure.
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