Topic: Practical Training
Podium Presentation in Room 6 on Thursday at 9:00 (Chair: Will Thompson / Judy Stone)
Authors: J. Will Thompson (1), Chris Shuford (2), Michael J. MacCoss (3)
Although most clinical laboratories perform system suitability testing (SST) prior to
performing analyses, most often the evaluation of these SST data is not quantitative,
and may not be tracked longitudinally. Quality Control (QC) metrics of a sample
batch, additionally, is typically only performed after completion of the study batch.
The combination of Skyline and Panorama workflows allow real time, quantitative
evaluation of SST and QC data to quickly identify system problems or batches that
fail; utilizing this resource routinely should lead to improved system and laboratory uptime.
Take home "Pearls" *
1. Setup of Skyline for any small molecule or peptide targets of interest;
2. How to utilize Skyline for quantitative evaluation of retention time, peak
area, and peak shape;
3. How to enable AutoQC and Panorama along with control charts to perform
SST evaluation and real-time QC.
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