MSACL 2022 Abstract
Self-Classified Topic Area(s): Imaging > Multi-omics > Emerging Technologies
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Podium Presentation in De Anza 2 on Wednesday at 15:35 (Chair: Peggi Angel / James Weatherill)
 Mass Spectrometry Histochemistry of Human FFPE Material Getting Ready for the Clinic
Peter Verhaert (1); Kenneth Verheggen (1); Nivedita Bhattacharya (1); Remco Crefcoeur (1); Samruddhi Chawan (1,2), Yury Tsybin (3); Konstantin Nagornov (3); Gilles Frache (4); Cecilia Lindskog-Bergström (5); Emma Lundberg (5); Per Andrén (6); Marthe Verhaert (1,2,7); Sandrine Aspeslagh (2); Raf Sciot (7); Kris Van Der Steen (8); Lin Zhang (9); Ling Shan (9); Dick Swaab (9); Wim Develter (10); Marc Ramael (10)
(1) ProteoFormiX, Beerse, Belgium
(2) University Hospital, Jette (Brussels), Belgium
(3) Spectroswiss, Lausanne, Switzerland
(4) Luxembourg Institute of Science and Technology, Esch-sur-Alzette, Luxembourg
(5) Human Protein Atlas, Uppsala & Stockholm, Sweden
(6) Science for Life Labs, Uppsala Sweden
(7) University Hospital, Leuven, Belgium
(8) Onze-Lieve-Vrouw Ziekenhuis, Aalst, Belgium
(9) The Netherlands Institute for Neuroscience, Amsterdam, the Netherlands
(10) Heilig-Hart Ziekenhuis, Lier, Belgium
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Peter Verhaert (Presenter)
ProteoFormiX |
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Presenter Bio: Peter VERHAERT was full professor Analytical Biotechnology and Innovative Peptide Biology at Delft University of Technology [Netherlands; 2005 – 2016] before founding his company ProteoFormiX (www.proteoformix.com). His Flemish research startup resides at JLABS@BE, the J&J Innovation Center on the Campus of Janssen Pharmaceuticals in Beerse [Belgium; 2017].
Peter is recognized worldwide as one of the pioneers in Peptidomics, Peptides in Biology being the common theme in his >35 years of research.
Prior to taking up his academic position in Delft and ultimately starting his own research company in Beerse, Peter's international career already combined academic and industrial positions:
He obtained an MSc in Biology (Zoology Group) and a PhD in Comparative Neurobiology at the University of Leuven, where he held a position as assistant professor [Belgium; 1983-1987].
He then took on a postdoc position at the Laboratory of Toxicology and Biochemistry in the Biology Department of the University of Waterloo [Canada; 1988].
Upon returning to the University of Leuven, he became an associate professor in Histology and Biological Mass Spectrometry [Belgium; 1988-1999].
After an industrial sabbat year at the Flemish Biotech Innogenetics in Ghent [Belgium; 1998-1999], Peter was appointed group leader Proteomics at the Dutch Pharma company Organon (now MSD) in Oss [Netherlands; 2000-2004].
His other professional activities include:
• Co-founder and president of the European Pharmaceutical Proteomics Laboratories (EPPL) [2000-2004];
• Proteomics expert at the Flemish Institute of Biotechnology (VIB) [2000-2004];
• Visiting professor Biomedical Proteomics at the Biomedical Research Institute at the Faculty of Medicine of Hasselt University [Belgium; 2004-2014];
• Editor-in-chief of the Elsevier Open Access Journal EuPA Open Proteomics [2013-2016]
• Co-director of the Center of Excellence in Biomedical Mass Spectrometry (CEBMMS) at the Faculty of Medicine (Clinical Sciences Department) of Lund University [Sweden; 2016];
• Appointment as honorary professor Mass Spectrometry Histochemistry at the Maastricht Multimodal Molecular Imaging Institute (M4i) of Maastricht University [Netherlands; 2017].
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Abstract Introduction
In our research facility at JLABS@BE, we run a platform to discover candidate biomarker molecules for diseases with a high medical need. Our analytical workflow uses histological slides of formalin-fixed paraffin-embedded (FFPE) tissue straight from the clinic. Biomarker molecules are identified, with simultaneous mapping of their location on the tissue section. In analogy with immunohistochemistry, this imaging method is designated mass spectrometry histochemistry (MSHC). With MSHC we explore the vast collections of well-documented human neuronal tissues conserved in world famous tissue banks, as well as in modern hospital pathology archives.
By mapping all FFPE detectable biomolecules (particularly peptides, neurotransmitters and metabolites), we are compiling the FFPE Biomolecular Atlas of the Human Body.
Methods
Next to the large availability of FFPE samples and their extraordinary stability, a major advantage of MSHC is that it is a completely untargeted technique which directly links all existing histopathology knowledge with novel biomolecular information.
We have modified and fitted a high-resolution MALDI source (MassTech AP MALDI UHRTM) with a high-resolution FTMS analyzer (ThermoFisher Scientific LTQ Orbitrap VelosTM).
MSHC performance is benchmarked employing sections from biobanked human 'model' tissues.
Tissue blocks representing relevant Homo sapiens diseases are sectioned on a regular microtome at 5 µm thickness and deposited on regular microscope glass slides.
Slides are coated with a spray of DHB using an automated spraying device (HTX TM5TM Sprayer). Multi-Gb MSHC data files are processed, analyzed, and visualized using MozaicTM software (Spectroswiss).
Preliminary Data
MSHC allows routine imaging of biomolecules, including neuropeptides as well as biogenic amines and metabolites with high mass accuracy and 20 µm lateral resolution (between 5 and 10 µm can be achieved).
Data of reference secretory peptides (e.g., the disulfide bridge-linked neuropeptides vasopressin and oxytocin in pituitary/hypothalamus), establish MSHC as single cell 'multi-omics' technology. Multi-Gb MSHC data sets from biopsy as well as autopsy samples of patients exhibiting various stages histologically diagnosed diseases with high unmet needs are currently explored with high priority.
We warmly welcome interested collaborators to our Homo sapiens FFPE Biomolecular Body Atlas project.
Novel Aspect
Biomolecule mapping of secretory peptides and metabolites on histological sections of human FFPE clinical tissue representing health and disease.
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Financial Disclosure
| Description | Y/N | Source |
| Grants | yes | European Institute of Innovation & Technology EIT) - Health |
| Salary | no | |
| Board Member | yes | ProteoFormiX; Lake Louise Tandem Mass Spectrometry Organizing Committee |
| Stock | no | |
| Expenses | no | |
| IP Royalty | no | |
| Planning to mention or discuss specific products or technology of the company(ies) listed above: |
yes |
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= Emerging. More than 5 years before clinical availability. (19.79%, 2022)
= Clinically available now. (42.25%, 2022)
