Advanced mass spectrometry (MS) has broadly grown in tissue sample analysis, namely, imaging mass spectrometry (IMS), imaging mass cytometry (IMC), high-throughput analysis of tissue microarrays using automated desorption electrospray ionization mass spectrometry (DESI-MS), MasSpec pen, intelligent knife (iKnife), Laser Desorption Probes (Handheld probes), and topography molecular imaging with the assistance of a robotic arm coupled with water-assisted laser desorption (SpiderMass). However, intraoperative applications of MS require to be introduced as routine techniques for pathologists to improve patient healthcare and to extend the assessment of decision-making skills for surgeons.
The bold ambition is to promote MS to the broader clinical community, integrating with quantitative multimodal imaging to visualize the data hidden in tissue samples. Because in a clinical setting, the primary concern is to analyze thin tissue sections from patients after sample preparation which could negatively affect sensitivity analysis of assurance methods and imaging quality. This is so we can link IMS and or IMC techniques to other imaging platforms, allowing interactions with pathologists, image analysis scientists, and surgeons in biomarker discovery, immuno-oncology, neuroscience, and metabolism.
Outline an IMS infographic, integrating imaging tools such as HALO and SciLS Lab, providing an understanding of quantitative multimodal imaging with simultaneous histological assessment.
The infographic IMS project incorporated educational technology and engaged the multi-disciplinary team (chemists, pathologists, and surgeons) in clinical laboratories. Several beneficial features, such as a quick overview of IMS technology in the clinical setting and multimodal imaging integration, could promote visual literacy and develop creativity in the imaging domain.
The infographic IMS project helps clinicians understand the world of multimodal imaging workflows for later use at the operating suite housed in a hospital to aid pathologists and surgeons in exploring emerging questions in immunology, neuroscience, and cancer.